|
Toll Free: (877) 796-6397 -- USA and Canada only -- |
Fax: +1-832-582-8590 Orders: +1-832-582-8158 |
Tech Support: +1-832-582-8158 Ext:3 Please provide your Order Number in the email. |
Technical Data
| Formula | C21H21NO.HCl |
|||
| Molecular Weight | 339.86 | CAS No. | 157654-67-6 | |
| Solubility (25°C)* | In vitro | |||
|
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
||||
Biological Activity
| Description | NSC 632839 is not only a DUB inhibitor, but also a deSUMOylase inhibitor, it inhibits USP2, USP7, and SENP2 with EC50 of 45 μM, 37 μM, and 9.8 μM, respectively. | ||||||
|---|---|---|---|---|---|---|---|
| Targets |
|
||||||
| In vitro | NSC 632839 inhibits purified USP2- and USP7-mediated cleavage of Ub-PLA2. NSC 632839 does not inhibit the reporter enzyme PLA2 over the concentration range tested (1.2–150 μM), indicating that the reported inhibition is selective for isopeptidases. [1] NSC 632839 is capable of sustaining caspase-3/caspase-7 activity in the absence of a functional caspase-9 in E1A and E1A/C9DN cells. NSC 632839 induces apoptosis with IC50 of 15.65 μM and 16.23 μM in E1A and E1A/C9DN cells, respectively. NSC-632839 (10 μM) provokes the accumulation of polyubiquitins in E1A cells expressing HA-tagged ubiquitin. NSC-632839 (10 μM) dramatically increases the levels of Noxa and Mcl-1 in E1A cells during a time course analysis. NSC-632839 (10 μM) provokes mitochondrial fragmentation in a higher percentage of cells with an intact outer mitochondrial membrane compared with etoposide. [2] | ||||||
| Features | Less potent than NSC 144303 in inducing apoptosis of E1A and E1A/C9DN cells. | ||||||
| Density | g/mL | ||||||
Protocol (from reference)
References
|
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.