Nivolumab (anti-PD-1)

Catalog No.A2002        Batch: A200206

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Technical Data

CAS No. 946414-94-4
Formulation 100 mM Pro-Ac, 20 mM Arg, pH5.0
Isotype Human IgG4
Source CHO cells
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity 99.52%
Protein concentration 4.95mg/ml
Endotoxin Level ≤1 EU/mg

Biological Activity

Description Nivolumab (anti-PD-1) is a genetically engineered, fully human immunoglobulin (Ig) G4 monoclonal antibody directed against the negative immunoregulatory human cell surface receptor programmed death-1 (PD-1,PCD-1) with immune checkpoint inhibitory and antineoplastic activities. MW : 143.597 KD.
Targets
PD-1/PD-L1 interaction [2]
(Cell-free assay)		 PD-1/PD-L2 interaction [2]
(Cell-free assay)
2.52 nM 2.59 nM
In vitro

The cell binding ratio of 125I-Nivolumab and 125I-Nivolumab-DTPA to PD-1 is 13.78 ± 2.90% and 10.22 ± 2.62%, respectively. Conjugating of DTPA does not affect targeting of Nivolumab to PD-1 in vitro.[1]
In vivo

In SPECT images, lesions with high 99mTc-DTPA-nivolumab uptake and relatively clear background are shown at 6 h. Thereafter, the suspected intestinal thickening in Gd-free T1WI is observed at 2 h after the addition of Gd-DTPA-nivolumab.[1]

Protocol (Only for Reference)

Cell Assay:

  • Splenocytes were isolated from the spleens obtained from humanized PD-1 over-expressing mice following standard protocol. 125I labeled Nivolumab and Nivolumab-DTPA, respectively. To perform co-culture, 125I-Nivolumab and 125I-Nivolumab-DTPA (0.37 MBq/µg) were added to the freshly isolated splenocytes (1×106 cells) grown in 6-well plates, respectively. After incubation for 12 h at 4°C, cells were collected after rinsing three times with PBS. 125I radioactivity was determined in a gamma counter to evaluate their binding efficiency to PD-1.
Animal Study:

  • Animal Models: CRC mouse models (highly expressed human PD-1 antigen)
    Dosages: 11.1 MBq/10 μg
    Administration: i.v.
    Reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8883027/
     

Customer Product Validation

Data from [Data independently produced by , , Clin Cancer Res, 2019, doi:10.1158/1078-0432]

Apoptosis rates of Jurkat cells co-cultured for 72 h with H1975, A549 cells or H1975 with 10 μg/mL nivolumab were analyzed by Annexin V-FITC/PI assay (Left) and quantified (Right).

Data from [Data independently produced by , , Ann Surg Oncol, 2019, 26(1):139-147]

Preliminary immunotherapy drug screening using immune checkpoint inhibitors pembrolizumab and nivolumab in LGA organoids immune-enhanced with cells from a lymph node from the same patient. Mitochondrial metabolism was assessed at (c) 24 h and (d) 96 h after administration of the immunotherapy agents. Statistical significance: **p<0.05 between tumor cell-only and immune-enhanced organoids; p<0.05 between drug treatment and control of the same group.

Selleck's Nivolumab (anti-PD-1) has been cited by 81 publications

Clinical drug screening reveals clofazimine potentiates the efficacy while reducing the toxicity of anti-PD-1 and CTLA-4 immunotherapy [ Cancer Cell, 2024, S1535-6108(24)00080-1] PubMed: 38518774
Base editing screens define the genetic landscape of cancer drug resistance mechanisms [ Nat Genet, 2024, 10.1038/s41588-024-01948-8] PubMed: 39424923
Human papillomavirus-encoded circular RNA circE7 promotes immune evasion in head and neck squamous cell carcinoma [ Nat Commun, 2024, 15(1):8609] PubMed: 39366979
Immunologic signatures of response and resistance to nivolumab with ipilimumab in advanced metastatic cancer [ J Exp Med, 2024, 221(10)e20240152] PubMed: 39190534
Assessing personalized responses to anti-PD-1 treatment using patient-derived lung tumor-on-chip [ Cell Rep Med, 2024, S2666-3791(24)00241-6] PubMed: 38703767
Genomic and transcriptomic profiling of peripheral T cell lymphoma reveals distinct molecular and microenvironment subtypes [ Cell Rep Med, 2024, 5(2):101416] PubMed: 38350451
Patient-derived follicular lymphoma spheroids recapitulate lymph node signaling and immune profile uncovering galectin-9 as a novel immunotherapeutic target [ Blood Cancer J, 2024, 14(1):75] PubMed: 38697976
Patient-derived follicular lymphoma spheroids recapitulate lymph node signaling and immune profile uncovering galectin-9 as a novel immunotherapeutic target [ Blood Cancer J, 2024, 14(1):75] PubMed: 38697976
NKG2A+CD8+ T cells infiltration determines immunosuppressive contexture and inferior response to immunotherapy in clear cell renal cell carcinoma [ J Immunother Cancer, 2024, 12(1)e008368] PubMed: 38262706
EGFR mutations induce the suppression of CD8+ T cell and anti-PD-1 resistance via ERK1/2-p90RSK-TGF-β axis in non-small cell lung cancer [ J Transl Med, 2024, 22(1):653] PubMed: 39004699

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

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