(-)-Arctigenin

Catalog No.S3897 Batch:S389701

Technical Data

Formula	C₂₁H₂₄O₆
Molecular Weight	372.41	CAS No.	7770-78-7
Solubility (25°C)*	In vitro	DMSO	74 mg/mL (198.7 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	(-)-Arctigenin, a lignan found in certain plants of the Asteraceae, exerts biological activities including anti-inflammatory, antiviral and anticancer.
In vitro	Arctigenin induces apoptosis in HT-29 colon cancer cells by regulating ROS and p38 MAPK pathways^[1]. It is a phytoestrogen that exerts a proliferative effect by binding to the ER. Treatment with arctigenin effectively downregulates ERα expression. Thus, arctigenin, mainly targets the mTOR pathway in ERα-positive MCF-7 human breast cancer cells, leading to autophagy-induced cell death and the downregulation of ERα expression^[2]. Arctigenin decreases BACE1 translation via dephosphorylation of eIF2α, causing the suppression of Aβ production, and initiates autophagy through both AMPK/Raptor signaling activation and AKT/mTOR pathway inhibition, resulting in enhanced Aβ clearance. Arctigenin suppresses BACE1 activity via inhibition of BACE1 translation^[3].
In vivo	Treatment of arctigenin in mice highly decreases Aβ formation and senile plaques and efficiently ameliorates AD mouse memory impairment of APP/PS1 transgenic model mice^[3].

Protocol (from reference)

Cell Assay:^{^[3]}	Cell lines HEK293-APP_swe, primary cortical neuronal, and BV2 cells Concentrations 0.1, 1, 10, 20, 40 μM Incubation Time 24 h Method Cells are treated with 0.1% DMSO or different concentrations of arctigenin for 24 h. The cell culture media are collected, and a complete protease inhibitor mixture is added. The culture media are then centrifuged by 20,000 × g at 4°C for 30 min, and supernatants are collected.
Animal Study:^{^[3]}	Animal Models The APP/PS1 mice Dosages 3 mg/kg/d Administration i.p.

Cell Assay:^{^[3]}

Cell lines
HEK293-APP_swe, primary cortical neuronal, and BV2 cells
Concentrations
0.1, 1, 10, 20, 40 μM
Incubation Time
24 h
Method
Cells are treated with 0.1% DMSO or different concentrations of arctigenin for 24 h. The cell culture media are collected, and a complete protease inhibitor mixture is added. The culture media are then centrifuged by 20,000 × g at 4°C for 30 min, and supernatants are collected.

Animal Study:^{^[3]}

Animal Models
The APP/PS1 mice
Dosages
3 mg/kg/d
Administration
i.p.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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