Napabucasin (BBI608)

Catalog No.S7977 Batch:S797702

Technical Data

Formula	C₁₄H₈O₄
Molecular Weight	240.21	CAS No.	83280-65-3
Solubility (25°C)*	In vitro	DMSO	14 mg/mL (58.28 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Napabucasin (BBI608) is an orally available Stat3 and cancer cell stemness inhibitor.

Targets

Stat3 ^[1]

In vitro

Napabucasin downregulates stemness gene expression driven by Stat3 and cancer stemness properties, and effectively inhibits self-renewal of stemness-high cancer cells with IC50 ranged from 0.291~1.19 μM, on inhibition on normal stem cells. ^[1]

In vivo

In mice bearing PaCa-2 xenografts, Napabucasin (20 mg/kg, i.p.) significantly inhibits tumor growth, relapse and metastasis. ^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Cancer stem cells U87-MG, U118, COLO205, DLD1, SW480, HCT116, FaDu, ACHN, SNU-475, Huh7, HepG2, H1975, A549, H460, CAOV-3, SW-626, PaCa2. Concentrations ~2 μM Incubation Time 72 h Method For cancer stem cells, spheres are dissociated and plated under cancer stem cell culture conditions on coated 96-well plates. After 72 h of culture, wells are dosed with the indicated compounds. Seventy-two hours or 24 h after dosing, CellTiter-Glo 2.0 is added to each well, and the luminescence is measured as described by the manufacturer. IC50 values are calculated by fitting a four parameter dose–response curve to normalized data using GraphPad Prism software. For bulk cells, cells are plated at 5,000 cells per well on 96-well plates. Twenty-four hours after plating, cells are treated with the indicated compounds. Viability is determined at 72 h as described above.
Animal Study:^{^[1]}	Animal Models Mice bearing PaCa-2 xenografts Dosages 20 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
Cancer stem cells U87-MG, U118, COLO205, DLD1, SW480, HCT116, FaDu, ACHN, SNU-475, Huh7, HepG2, H1975, A549, H460, CAOV-3, SW-626, PaCa2.
Concentrations
~2 μM
Incubation Time
72 h
Method
For cancer stem cells, spheres are dissociated and plated under cancer stem cell culture conditions on coated 96-well plates. After 72 h of culture, wells are dosed with the indicated compounds. Seventy-two hours or 24 h after dosing, CellTiter-Glo 2.0 is added to each well, and the luminescence is measured as described by the manufacturer. IC50 values are calculated by fitting a four parameter dose–response curve to normalized data using GraphPad Prism software. For bulk cells, cells are plated at 5,000 cells per well on 96-well plates. Twenty-four hours after plating, cells are treated with the indicated compounds. Viability is determined at 72 h as described above.

Animal Study:^{^[1]}

Animal Models
Mice bearing PaCa-2 xenografts
Dosages
20 mg/kg
Administration
i.p.

References

[1] Li Y, et al. Proc Natl Acad Sci U S A. 2015, 112(6), 1839-1844.

Selleck's Napabucasin (BBI608) has been cited by 29 publications

Serum amyloid A promotes glycolysis of neutrophils during PD-1 blockade resistance in hepatocellular carcinoma [ Nat Commun, 2024, 15(1):1754]	PubMed: 38409200
Molecular crosstalk between MUC1 and STAT3 influences the anti-proliferative effect of Napabucasin in epithelial cancers [ Sci Rep, 2024, 14(1):3178]	PubMed: 38326371
ADAR1-mediated RNA editing of SCD1 drives drug resistance and self-renewal in gastric cancer [ Nat Commun, 2023, 14(1):2861]	PubMed: 37208334
Targeting VCP potentiates immune checkpoint therapy for colorectal cancer [ Cell Rep, 2023, 42(11):113318]	PubMed: 37865914
Alcohol reshapes a liver premetastatic niche for cancer by extra- and intrahepatic crosstalk-mediated immune evasion [ Mol Ther, 2023, 31(9):2662-2680]	PubMed: 37469143
Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells [ BioRxiv, 2023, 10.1101/2023.06.19.545595]	PubMed: none
CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states [ Nat Neurosci, 2022, 25(11):1528-1542]	PubMed: 36303069
Ligand-mediated PAI-1 inhibition in a mouse model of peritoneal carcinomatosis [ Cell Rep Med, 2022, 3(2):100526]	PubMed: 35243423
Targeted inhibition of STAT3 induces immunogenic cell death of hepatocellular carcinoma cells via glycolysis [ Mol Oncol, 2022, 16(15):2861-2880]	PubMed: 35665592
Identification of Novel Therapeutic Targets for Fibrolamellar Carcinoma Using Patient Derived Xenografts and Direct from Patient Screening [ Cancer Discov, 2021, candisc.0872.2020]	PubMed: 34127480

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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