m6A (N6-methyladenosine)

Catalog No.S3190 Batch:S319009

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Technical Data

Formula

C11H15N5O4
Molecular Weight 281.27 CAS No. 1867-73-8
Solubility (25°C)* In vitro DMSO 56 mg/mL (199.09 mM)
Water 14 mg/mL (49.77 mM)
Ethanol 9 mg/mL (31.99 mM)
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
0.35mg/ml Taking the 1 mL working solution as an example, add 50 μL of 7 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
2.8mg/ml Taking the 1 mL working solution as an example, add 50 μL of 56 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description m6A (N6-methyladenosine) is a base modified analog of adenosine and is found as a minor nucleoside in natural RNAs.
In vitro N6-Methyladenosine is an abundant modification in mRNA and is found within some viruses, and most eukaryotes including mammals, insects, plants and yeast. It is also found in tRNA, rRNA, and small nuclear RNA(snRNA) as well as several long non-coding RNA, such as Xist. [1] [2] [3] N6-Methyladenosine is an endogenous urinary nucleoside product of the degradation of transfer ribonucleic acid (tRNA). [4] N6-Methyladenosine is a widespread RNA modification in many tissues with high levels in the brain. N6-Methyladenosine is enriched near stop codons and within 3’UTRs in both mouse and human mRNAs. [5] The recent discovery that FTO, an obesity risk gene, encodes an m6A demethylase implicates m6A as an important regulator of physiological processes. [6]
In vivo LD50: Mice >1g/kg (i.g.). [7]

Protocol (from reference)

Customer Product Validation

, , Biochem Biophys Res Commun, 2016, 476(4):340-5.

Selleck's m6A (N6-methyladenosine) has been cited by 22 publications

A low-cost, low-input method establishment for m6A MeRIP-seq [ Biosci Rep, 2024, 44(1)BSR20231430] PubMed: 38113135
The S. cerevisiae m6A-reader Pho92 promotes timely meiotic recombination by controlling key methylated transcripts [ Nucleic Acids Res, 2023, 51(2):517-535] PubMed: 35934316
METTL3/YTHDF2 m6A axis promotes the malignant progression of bladder cancer by epigenetically suppressing RRAS [ Oncol Rep, 2023, 49(5)94] PubMed: 36960869
Autophagy induction promoted by m6A reader YTHDF3 through translation upregulation of FOXO3 mRNA [ Nat Commun, 2022, 13(1):5845] PubMed: 36195598
The S. cerevisiae m6A-reader Pho92 promotes timely meiotic recombination by controlling key methylated transcripts [ Nucleic Acids Res, 2022, gkac640] PubMed: 35934316
Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex [ Cell Rep, 2022, 40(3):111107] PubMed: 35858564
The Alteration of m6A Modification at the Transcriptome-Wide Level in Human Villi During Spontaneous Abortion in the First Trimester [ Front Genet, 2022, 13:861853] PubMed: 35754822
Overexpression of m6A-factors METTL3, ALKBH5, and YTHDC1 alters HPV16 mRNA splicing [ Virus Genes, 2022, 58(2):98-112] PubMed: 35190939
NAR Breakthrough Article The S. cerevisiae m 6 A-reader Pho92 promotes timely [ HAL Open Science, 2022, 10.1093/nar/gkac640] PubMed: None
Small-Molecule Ebselen Binds to YTHDF Proteins Interfering with the Recognition of N 6-Methyladenosine-Modified RNAs [ ACS Pharmacol Transl Sci, 2022, 5(10):872-891] PubMed: 36268123

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.