Mogroside V

Catalog No.S3815 Batch:S381501

Technical Data

Formula	C₆₀H₁₀₂O₂₉
Molecular Weight	1287.43	CAS No.	88901-36-4
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (77.67 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Mogroside V is a natural cucurbitane glycoside which has a sweetening strength of 250 times that of sucrose and is derived from mature fruit of luo-han-guo (Siraitia grosvenorii, monk fruit).
In vitro	Mogroside V possesses tumor growth inhibitory activity in in vitro and in vivo models of pancreatic cancer by promoting apoptosis and cell cycle arrest of pancreatic cancer cells (PANC-1 cells), which may in part be mediated through regulating the STAT3 signaling pathway. Mogroside V inhibits the proliferation of pancreatic cancer cells and other tumor cell types in a dose- and time-dependent manner, and shows very little cytotoxicity against the non-tumorigenic epithelial cell line L02. Mogroside V inhibits the downstream targets of the STAT3 pathway that promote cell proliferation (CCND1, CCNE1 and CDK2), while also upregulating cell cycle inhibitors (CDKN1A and CDKN1B)^[1].
In vivo	Mogroside V treatment promotes apoptosis of pancreatic cancer cells in the xenograft tumors. Mogroside V treatment significantly reduces the expression of CD31-labeled blood vessels and of the pro-angiogenic factor vascular endothelial growth factor in the xenografts, indicating that mogroside V might limit the growth of pancreatic tumors by inhibiting angiogenesis and reducing vascular density. Mogroside V treatment inhibits tumor cell growth, induces apoptosis and causes tumor regression^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines PANC-1 cells Concentrations 0 to 250 μmol/l Incubation Time 24 or 72 h Method Cells are added to 96-well cell culture plates at an initial concentration of 1 × 10⁴ cells/ml and are incubated with the indicated concentrations of mogroside V. After 24 or 72 h of incubation, MTT solution (5 mg/ml) is added to the wells and the cells are incubated for another 4 h. The growth medium is removed and formazan crystals formed by oxidation of the MTT dye are analyzed by measuring the absorbance at 490 nm using an ELISA reader.
Animal Study:^{^[2]}	Animal Models BALB/c nude mice xenografted with PANC-1 cells Dosages 2 mg/kg, 10 mg/kg, 30 mg/kg Administration i.v.

Cell Assay:^{^[1]}

Cell lines
PANC-1 cells
Concentrations
0 to 250 μmol/l
Incubation Time
24 or 72 h
Method
Cells are added to 96-well cell culture plates at an initial concentration of 1 × 10⁴ cells/ml and are incubated with the indicated concentrations of mogroside V. After 24 or 72 h of incubation, MTT solution (5 mg/ml) is added to the wells and the cells are incubated for another 4 h. The growth medium is removed and formazan crystals formed by oxidation of the MTT dye are analyzed by measuring the absorbance at 490 nm using an ELISA reader.

Animal Study:^{^[2]}

Animal Models
BALB/c nude mice xenografted with PANC-1 cells
Dosages
2 mg/kg, 10 mg/kg, 30 mg/kg
Administration
i.v.

References

[1] C Liu, et al. Oncogenesis. 2016, 5(4): e217.

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SHIPPING AND STORAGE
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