MLN120B

Catalog No.S7736 Batch:S773601

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Technical Data

Formula

C19H15ClN4O2
Molecular Weight 366.80 CAS No. 783348-36-7
Solubility (25°C)* In vitro DMSO 73 mg/mL (199.01 mM)
Ethanol 5 mg/mL (13.63 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
0.91mg/ml Taking the 1 mL working solution as an example, add 50 μL of 18.2 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
0.36mg/ml Taking the 1 mL working solution as an example, add 50 μL of 7.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description MLN120B (ML120B) is a potent, selective, reversible, and ATP-competitive inhibitor of IkappaB kinase beta (IKKβ) with an IC50 of 45 nM for recombinant IKKβ. MLN120B does not inhibit other IKK isoforms at IC50 below 50 μM.
Targets
recombinant IKKβ [1]
45 nM
In vitro

MLN120B, a anti-inflammatory compound, is a NF-kB pathway blocker of inhibiting IKK2, and shows an inhibitory effect on LPS induced NF-kB activation. The IC50 value of MLN120B is 1.4, 14.8 or 27.3 μM for NF-kB2-luc2, IL8-luc2 or TNF-AIP3-luc2 reporter transfected cells, respectively, indicating that IKK2 inhibition affects the transcriptional activity of the NF-kB2 promoter most effective.<sup><a class="sref" href="#s_ref">[1]</a></sup><sup><a class="sref" href="#s_ref">[2]</a></sup>
In vivo

Significantly decreased levels of the pro-inflammatory chemokine MIP-1β and increased levels of the anti-inflammatory cytokine IL-10 were detected in the BAL of MLN120B pre-treated mice as opposed to LPS-treated positive controls.<sup><a class="sref" href="#s_ref">[2]</a></sup>

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    TNFAIP3-luc2, NF-kB2-luc2 and IL8-luc2 transfected RAW264.7 cells

  • Concentrations

    1-100 µM

  • Incubation Time

    --

  • Method

    Transiently transfect the NF-kB reporters into RAW267.4 cells and induce inflammation in the cells with LPS. The effect of these compounds on LPS induced luciferase expression was assayed. 
Animal Study:

[2]
  • Animal Models

    NF-kB2-luc2 transfected mice

  • Dosages

    300 mg/kg

  • Administration

    p.o.

Selleck's MLN120B has been cited by 1 publication

Oxysterols contribute to immune cell recruitment in SLE skin lesions [ Arthritis Res Ther, 2024, 26(1):181] PubMed: 39438997

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.