ML364

Catalog No.S6748 Batch:S674803

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Technical Data

Formula

C24H18F3N3O3S2
Molecular Weight 517.54 CAS No. 1991986-30-1
Solubility (25°C)* In vitro DMSO 60 mg/mL (115.93 mM)
Ethanol 15 mg/mL (28.98 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description ML364 is a small molecule inhibitor of the deubiquitinase USP2 with an IC50 of 1.1 μM in a biochemical assay using an internally quenched fluorescent di-ubiquitin substrate.
Targets
USP8 [1]
(Cell-free assay)		 USP2 [1]
(Cell-free assay)
0.95 μM 1.1 μM
In vitro

ML364 induces an increase in cellular cyclin D1 degradation and causes cell cycle arrest in Mino and HCT116 cancer cell lines. ML364 is antiproliferative in cancer cell lines. ML364 also causes a decrease in homologous recombination-mediated DNA repair. It is inactive against caspase 6, caspase 7, MMP1, MMP9, and USP15, but does inhibit USP8 with an IC50 of 0.95 μM. In a panel of 102 kinases that included regulators of the cell cycle there is no binding observed to any of the enzymes tested using 10 μM ML364[1].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    LnCAP cells and MCF7 cells

  • Concentrations

    0-20 μM

  • Incubation Time

    24 h

  • Method

    ML364 promotes degradation of cyclin D1 in LnCAP cells and MCF7 cells. Cells are treated with ML364 for 24 h, and cyclin D1 protein levels are assessed by Western blotting using a tubulin control.

Selleck's ML364 has been cited by 2 publications

USP2 promotes experimental colitis and bacterial infections by inhibiting the proliferation of myeloid cells and remodeling the extracellular matrix network [ Cell Insight, 2022, 1(4):100047] PubMed: 37192862
Epstein-Barr virus-encoded latent membrane protein 1 and B-cell growth transformation induces lipogenesis through fatty acid synthase [ J Virol, 2020, JVI.01857-20] PubMed: 33208446

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.