Mitomycin C

Catalog No.S8146 Batch:S814602

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Technical Data

Formula

C15H18N4O5
Molecular Weight 334.37 CAS No. 50-07-7
Solubility (25°C)* In vitro DMSO 66 mg/mL (197.38 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Mitomycin C (Ametycine) is an antineoplastic antibiotic by inhibiting DNA synthesis, used to treat different cancers. Mitomycin C induces apoptosis in a caspases-dependent and Fas/CD95-independent manner.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
Targets
DNA synthesis [1]
In vitro

Mitomycin C, by inducing DNA interstrand crosslinks, physically blocks DNA replication, recombination, and RNA transcription. [1] Mitomycin C potentiates TRAIL-induced apoptosis in HCT116 (p53-/-) colon cancer cells and sensitizes TRAIL-resistant colon cancer cells HT-29 to the cytokine by through JNK-independent upregulation of death receptors. [2] In different human cancer cell lines, such OVCAR-5 (ovary), HT-29 (colon), SK-N-MC (neuroblastoma), HEP-2 (liver), COLO-205 (colon), NIH-OVCAR-3 (ovary) and A-549 (lung) cells, Mitomycin C shows cytotoxic activity. [3]
In vivo

As the clinical first choice in superficial bladder tumors, in a rat bladder tumor model, Mitomycin C (400 μM) significantly prevents intravesical tumor growth. [4]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    HCT116 cells

  • Concentrations

    5 µM

  • Incubation Time

    24 h

  • Method

    Cells were pre-treated with 5 µM MMC for 24 h and then co-treated with TRAIL (25 ng/mL).
Animal Study:

[4]
  • Animal Models

    Rat bladder tumor model

  • Dosages

    400 μM

  • Administration

    Intravesical treatment

Customer Product Validation

Data from [Data independently produced by , , Cancer Sci, 2018, 109(11):3450-3460]

Selleck's Mitomycin C has been cited by 129 publications

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High-throughput drug screening identifies novel therapeutics for Low Grade Serous Ovarian Carcinoma [ Sci Data, 2024, 11(1):1024] PubMed: 39300112
CCR5-overexpressing mesenchymal stem cells protect against experimental autoimmune uveitis: insights from single-cell transcriptome analysis [ J Neuroinflammation, 2024, 21(1):136] PubMed: 38802924
Elucidation of the pluripotent potential of bovine embryonic lineages facilitates the establishment of formative stem cell lines [ Cell Mol Life Sci, 2024, 81(1):427] PubMed: 39377807
Culture and expansion of murine proximal airway basal stem cells [ Stem Cell Res Ther, 2024, 15(1):26] PubMed: 38287366
Establishment, characterization, and biobanking of 36 pancreatic cancer organoids: prediction of metastasis in resectable pancreatic cancer [ Cell Oncol (Dordr), 2024, 10.1007/s13402-024-00939-5] PubMed: 38619751
Enhanced anti-tumor efficacy of electroporation (EP)-mediated DNA vaccine boosted by allogeneic lymphocytes in pre-established tumor models [ Cancer Immunol Immunother, 2024, 73(12):248] PubMed: 39358555
Arabidopsis Histone Variant H2A.X Functions in the DNA Damage-Coupling Abscisic Acid Signaling Pathway [ Int J Mol Sci, 2024, 25(16)8940] PubMed: 39201623
The photoreactivation of 6 - 4 photoproducts in chloroplast and nuclear DNA depends on the amount of the Arabidopsis UV repair defective 3 protein [ BMC Plant Biol, 2024, 24(1):723] PubMed: 39080534

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.