Militarine

Catalog No.E0247 Batch:E024701

Technical Data

Formula	C₃₄H₄₆O₁₇
Molecular Weight	726.72	CAS No.	58139-23-4
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (137.6 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Militarine is a major chemical constituent of the tuber of Bletilla striata (Thunb.) Reichb.f., with a prominent neuroprotective effect.
In vitro	Militarine remarkably decreases in IL-6, TNF-α, cyclooxygenase-2 (COX-2) and interleukin-1β (IL-1β) mRNA levels, phospho-IκB kinase β/IκB kinase β (p-IKKβ/IKKβ), phospho-nuclear factor of kappa B p65/nuclear factor of kappa B p65 (p-NF-κBp65/NF-κBp65) and COX-2 proteins, concomitant with a significant increase of expression of inhibitor of NF-κB (IκBα) protein in PM 2.5-exposed human lung alveolar epithelial A549 cells.^[2]
In vivo	Militarine promotes rehabilitation of white matter, and increases levels of myelin basic protein (MBP), also markedly suppresses loss of CNPase-positive oligodendrocytes in rats with chronic cerebral hypoperfusion.^[1]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines PM 2.5-exposed human lung alveolar epithelial A549 cells Concentrations 1.25, 2.5, 5, 10 μg/ml Incubation Time 2 h Method Cells are pretreated with coelonin (2.5, 5, 10, 20 μg/ml) or militarine (1.25, 2.5, 5, 10 μg/ml) for 2 h, followed by stimulation with PM2.5 (250 μg/ml) for 18 h. After treatment, the culture medium is centrifuged at 16,099 g for 10 min at 4℃ to separate the supernatant fraction. IL-6 and TNF-α levels are measured using the enzyme-linked immunosorbent assay according to the manufacturer s instructions.

Cell Assay:^{^[2]}

Cell lines
PM 2.5-exposed human lung alveolar epithelial A549 cells
Concentrations
1.25, 2.5, 5, 10 μg/ml
Incubation Time
2 h
Method
Cells are pretreated with coelonin (2.5, 5, 10, 20 μg/ml) or militarine (1.25, 2.5, 5, 10 μg/ml) for 2 h, followed by stimulation with PM2.5 (250 μg/ml) for 18 h. After treatment, the culture medium is centrifuged at 16,099 g for 10 min at 4℃ to separate the supernatant fraction. IL-6 and TNF-α levels are measured using the enzyme-linked immunosorbent assay according to the manufacturer s instructions.

References

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.