Miglustat

Catalog No.E1029 Batch:E102901

Technical Data

Formula	C₁₀H₂₁NO₄
Molecular Weight	219.28	CAS No.	72599-27-0
Solubility (25°C)*	In vitro	DMSO	44 mg/mL (200.65 mM)
		Water	44 mg/mL (200.65 mM)
		Ethanol	22 mg/mL (100.32 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Miglustat (Zavesca, N‐butyldeoxynojirimycin, OGT 918) is an orally administered ceramide glucosyltransferase inhibitor which prevents the lysosomal accumulation of glucocerebroside.

Targets

ceramide glucosyltransferase ^[1]

In vitro

Both HCT116 and Lovo cells show decreased tumor microsphere growth with pronounced growth retardation of Lovo cell spheroids at low concentrations of Miglustat. A marked elevation of SM and PC in is observed in Lovo cells treated with Miglustat.^[2]

In vivo

Miglustat treatment of Mcoln1^−/− mice successfully delays impairments in cerebellar-mediated motor coordination, Purkinje cell death, and cerebellar microgliosis.^[3]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines HCT116, Lovo cells Concentrations 100 µM Incubation Time 4 days Method Lovo and HCT116 cells are cultivated in MEM-alpha medium, cells are cultivated in 10 mL culture medium in- or without the presence of 100 µM Miglustat. Cells are cultivated in 10 cm tissue culture dishes for 4 days. Cells are counted, split and treated for another 2 days, each in triplicates. Cells are harvested and used for FACS-, protein-, and TLC-analysis.
Animal Study:^{^[3]}	Animal Models Mcoln1^−/− mice Dosages 300 mg/kg Administration i.p.

Cell Assay:^{^[2]}

Cell lines
HCT116, Lovo cells
Concentrations
100 µM
Incubation Time
4 days
Method
Lovo and HCT116 cells are cultivated in MEM-alpha medium, cells are cultivated in 10 mL culture medium in- or without the presence of 100 µM Miglustat. Cells are cultivated in 10 cm tissue culture dishes for 4 days. Cells are counted, split and treated for another 2 days, each in triplicates. Cells are harvested and used for FACS-, protein-, and TLC-analysis.

Animal Study:^{^[3]}

Animal Models
Mcoln1^−/− mice
Dosages
300 mg/kg
Administration
i.p.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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