MG-101 (ALLN)

Catalog No.S7386 Batch:S738602

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Technical Data

Formula

C20H37N3O4
Molecular Weight 383.53 CAS No. 110044-82-1
Solubility (25°C)* In vitro DMSO 5 mg/mL (13.03 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description MG-101 (ALLN, Calpain inhibitor-1, Ac-LLnL-CHO) is a cell-permeable and potent inhibitor of cysteine proteases including calpains and lysosomal cathepsins.
Targets
Cysteine protease [1]
In vitro MG-101 (ALLN) effectively inhibits cysteine proteinases with ID50 of 7 nM and 13 nM for cathepsins L and B, respectively. MG-101 (ALLN) shows very weak inhibitory activities towards cathepsin D (aspartic protease) and subtilisin (serine protease). MG-101 thus transforms NIH3T3 cells and also induces differentiation of PC12 pheochromocytoma cells. [1] MG-101, as an inhibitor of Ca(2+)-dependent cysteine proteases, inhibits the degradation of HMG-CoA reductase and HMGal in cholesterol biosynthesis. [2] In HCT116 cells, MG-101 decreases cell viability and tumor growth, and induces apoptosis response through Bax translocation from cytosol to mitochondria. [3]
In vivo In mice bearing HCT116 xenografts, MG-101 (10 mg/kg i.p.) inhibits colon tumor formation. [3]

Protocol (from reference)

Cell Assay:[3]
  • Cell lines

    HCT116, HCT116/p53−/−, and HCT116/Bax−/− cells

  • Concentrations

    ~26 μM

  • Incubation Time

    24 hours

  • Method

    Cells viability is assayed using Cell Counting Kit-8 following manufacturer’s protocol. All HCT116 cell typesare seeded into each well of 96-well plate, cultured to 80% density and treated with different doses of ALLN for 24 h. Medium is then replaced by 100 μl fresh McCoy’s 5A complete medium with 10% CCK-8 reagent and incubated for 1 h. Absorbance is measured at 450 nm using a microplate reader. Results are shown as death percentages.
Animal Study: [3]
  • Animal Models

    Female athymic nude mice bearing HCT116 xenografts

  • Dosages

    10 mg/kg

  • Administration

    i.p.

Customer Product Validation

Data from [Data independently produced by , , Graefes Arch Clin Exp Ophthalmol, 2018, 257(1):83-94]

Selleck's MG-101 (ALLN) has been cited by 20 publications

Targeting stress induction of GRP78 by cardiac glycoside oleandrin dually suppresses cancer and COVID-19 [ Cell Biosci, 2024, 14(1):115] PubMed: 39238058
CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression [ Exp Hematol Oncol, 2023, 12(1):77] PubMed: 37679762
CUL4B-DDB1-COP1-mediated UTX downregulation promotes colorectal cancer progression [ Exp Hematol Oncol, 2023, 12(1):77] PubMed: 37679762
Potential drug discovery for COVID-19 treatment targeting Cathepsin L using a deep learning-based strategy [ Comput Struct Biotechnol J, 2022, 20:2442-2454] PubMed: 35602976
Assembly and stability of IFT-B complex and its function in BBSome trafficking [ iScience, 2022, 25(12):105493] PubMed: 36411782
Nannocystin ax, an eEF1A inhibitor, induces G1 cell cycle arrest and caspase-independent apoptosis through cyclin D1 downregulation in colon cancer in vivo [ Pharmacol Res, 2021, S1043-6618(21)00454-0] PubMed: 34500061
Tunicamycin Induces Hepatic Stellate Cell Apoptosis Through Calpain-2/Ca2 +-Dependent Endoplasmic Reticulum Stress Pathway [ Front Cell Dev Biol, 2021, 9:684857] PubMed: 34604209
Tunicamycin Induces Hepatic Stellate Cell Apoptosis Through Calpain-2/Ca2 +-Dependent Endoplasmic Reticulum Stress Pathway [ Front Cell Dev Biol, 2021, 9:684857] PubMed: 34604209
SMAD-specific E3 ubiquitin ligase 2 promotes angiogenesis by facilitating PTX3 degradation in MSCs from patients with ankylosing spondylitis [ Stem Cells, 2021, 10.1002/stem.3332] PubMed: 33547700
Suppression of ER-stress induction of GRP78 as an anti-neoplastic mechanism of the cardiac glycoside Lanatoside C in pancreatic cancer: Lanatoside C suppresses GRP78 stress induction [ Neoplasia, 2021, 23(12):1213-1226] PubMed: 34768108

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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