Methyl gallate

Catalog No.S3790 Batch:S379001

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Technical Data

Formula

C8H8O5
Molecular Weight 184.15 CAS No. 99-24-1
Solubility (25°C)* In vitro DMSO 36 mg/mL (195.49 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Methyl Gallate (Methylgallate, Gallic acid methyl ester, Gallicin) is a plant polyphenol with antioxidant, anticancer, and anti-inflammatory activities.
In vitro Methyl gallate inhibits Treg cell-suppressive effects on effector CD4+ T cells and Treg migration toward tumor environment. The expression of Treg surface markers including CTLA-4, CCR4, CXCR4, and glucocorticoid-induced TNFR are significantly suppressed upon methyl gallate treatment[2].
In vivo In tumor-bearing hosts, methyl gallate treatment substantially reduces tumor growth and prolonged the survival rate. In contrast, nu/nu mice do not show decreased tumor progression in response to methyl gallate. In addition, in tumor-bearing Treg-depleted mice, tumor growth and the survival rates are not changed by methyl gallate treatment, strongly suggesting that the main therapeutic target of methyl gallate in tumor suppression is related to modulation of the CD4+CD25+ Treg cell functions. In the spleen of tumor-bearing mice, methyl gallate treatment induces a significant decrease in the CD4+CD25+Foxp3high Treg cell population. Especially, the number of tumor-infiltrating CD25+Foxp3high Treg cells is significantly lower in methyl gallate-treated mice. methyl gallate can be used to reverse immune suppression and as a potentially useful adjunct for enhancing the efficacy of immune-based cancer therapy[2].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    HUVEC

  • Concentrations

    0-0.5 mM

  • Incubation Time

    1 h

  • Method

    HUVECs are grown in 96-well micro plates in a final volume of 100 μl culture medium containing 104 cells per well. After allowing the cultures to grow to confluence, chemical and H2O2 treatments are followed with certain intervals. To determine the cell viability, XTT assay kit is used. Two cases of oxidative stress are applied to pre-incubated HUVECs with chemical for 1 h: the treatment with 1 mM H2O2 for 1 h and the treatment with 0.1 mM H2O2 for 48 h. Concentrations of chemical are varied from 0 to 0.5 mM.
Animal Study:[2]
  • Animal Models

    C57BL/6, Foxp3EGFPC57BL/6 or nu/nu mice

  • Dosages

    200 μg

  • Administration

    i.p.

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.