Methyl Dihydrojasmonate

Technical Data

Biological Activity

Protocol (from reference)

References

Catalog No.S5139 Batch:S513901

Formula	C₁₃H₂₂O₃
Molecular Weight	226.31	CAS No.	24851-98-7
Solubility (25°C)*	In vitro


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Cell Assay:^{^[1]}	Cell lines MCF-7 cells Concentrations 0, 5, 12.5, 25, 50 μl/ml Incubation Time 48 h Method Measurement of potential cytotoxicity is performed by SRB assay where Cells are plated in 96-multiwell plate (10⁴ cells/well) for 24 h before exposure to the compound(s) to allow attachment of the cells to the wall of the plate. Different concentrations of the compound under test; 0, 5, 12.5, 25, 50 μl/ml for the Pure MDHJ (Oily liquid) and 0, 25, 50, 75, 100 μl/ml for the used formulation are added to the cell monolayer triplicate wells; which are prepared for each individual concentration. A monolayer cells are incubated together with the compound(s) for 48 h at 37 °C and in atmosphere of 5% CO₂. After 48 h, cells are fixed, washed and stained with Sulfo-Rhodamine-B stain (SRB). Excess stain is washed with acetic acid and the attached stain is recovered with Tris EDTA buffer. Color intensity is measured utilizing an ELISA reader.
Animal Study:^{^[3]}	Animal Models Sprague-Dawley rats Dosages 0, 40, 80 or 120 mg/kg/day Administration oral

Cell Assay:^{^[1]}

Cell lines
MCF-7 cells
Concentrations
0, 5, 12.5, 25, 50 μl/ml
Incubation Time
48 h
Method
Measurement of potential cytotoxicity is performed by SRB assay where Cells are plated in 96-multiwell plate (10⁴ cells/well) for 24 h before exposure to the compound(s) to allow attachment of the cells to the wall of the plate. Different concentrations of the compound under test; 0, 5, 12.5, 25, 50 μl/ml for the Pure MDHJ (Oily liquid) and 0, 25, 50, 75, 100 μl/ml for the used formulation are added to the cell monolayer triplicate wells; which are prepared for each individual concentration. A monolayer cells are incubated together with the compound(s) for 48 h at 37 °C and in atmosphere of 5% CO₂. After 48 h, cells are fixed, washed and stained with Sulfo-Rhodamine-B stain (SRB). Excess stain is washed with acetic acid and the attached stain is recovered with Tris EDTA buffer. Color intensity is measured utilizing an ELISA reader.

Animal Study:^{^[3]}

Animal Models
Sprague-Dawley rats
Dosages
0, 40, 80 or 120 mg/kg/day
Administration
oral

Description

Methyl dihydrojasmonate (Hedione, Kharismal) is a flavouring ingredien with the smell vaguely similar to jasmin.

Methyl dihydrojasmonate inhibits the growth of leukemia cells as well as lung, breast, and prostate cancer cells. It opens the mitocondrial permeability transition-pore complex, allowing cytochrome C release and induction of apoptosis, and the expression of reactive oxygen species. Jasmonate-derivative compounds, including jasmonic acid (JA) and methyl dihydrojasmonate (MJ), also induces the suppression of cell proliferation in human and mouse cancer cell lines, such as prostate cancer and breast cancers, melanoma, leukemia, lymphoid, and myeloid^[2].

Methyl dihydrojasmonate (MDHJ) is a promising anti-cancer drug of a plant origin (jasmine plant)^[1]. Jasmonate-derivative compounds are poorly water-soluble, and cannot be administered intravenously or adequately absorbed by the body^[2]. The oral LD50 of Methyl dihydrojasmonate in rats exceeds 5.0 g/kg. By the dermal route, the rabbit LD50 also exceeds 5.0 g/kg. By the intraperitoneal route in ICR mice, the LD50 is 1397.2 mg/kg. While in rats, an intraperitoneal injection of Methyl dihydrojasmonate in corn oil produces clinical signs at 1000 mg/kg and mortality at 2000 mg/kg. Methyl dihydrojasmonate is not considered to possess a mutagenic or genotoxic potential^[3].

0.998 g/mL at 25 °C

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.