Methyl-β-cyclodextrin (MβCD)

Catalog No.S6827 Batch:S682701

Technical Data

Formula

Molecular Weight

CAS No.

128446-36-6

Solubility (25°C)*

In vitro

Water

100 mg/mL

In vivo (Add solvents to the product individually and in order)

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	5.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	2.5mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description

Methyl-β-cyclodextrin (MβCD) is a cholesterol-removing agent mainly used for lipid raft disruption. Methyl-β-cyclodextrin induces caspase-dependent apoptosis in PEL cells and inhibits the growth and invasion of PEL cells without apparent adverse effects, suggesting the potential effective antitumor activities.

Targets

cholesterol ^[1]

lipid raft ^[1]

In vitro

Methyl-β-cyclodextrin Inhibits SDF-1α-induced platelet aggregation and Akt phosphorylation by raft disruption.^[2]

In vivo

In a Primary effusion lymphoma (PEL) xenograft mouse model, Methyl-β-cyclodextrin significantly inhibits the growth and invasion of PEL cells without apparent adverse effects. Methyl-β-cyclodextrin-treated mice appears to be healthy, whereas non-treated mice has a distended abdominal region. The body weights of control are significantly higher than those of Methyl-β-cyclodextrin treated mice. Methyl-β-cyclodextrin-treated mice has a significantly lower volume of ascites than that of non-treated mice.^[1]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines Platelets Concentrations 2% Incubation Time 15 min Method Freshly isolated human platelets are incubated with SDF-1α and centrifuged for 1 min at 3000 rpm. The resulting pellet is resuspended and boiled in Laemmli sample buffer containing phosphatase inhibitor cocktails 2 and 3. Phosphorylations of Akt (Ser473) is detected by western blotting. Platelet aggregation is measured as the optical density of PRP during aggregation using a platelet aggregometer, with the platelet-poor plasma 100% standard for change in optical density.

Cell Assay:^{^[2]}

Cell lines
Platelets
Concentrations
2%
Incubation Time
15 min
Method
Freshly isolated human platelets are incubated with SDF-1α and centrifuged for 1 min at 3000 rpm. The resulting pellet is resuspended and boiled in Laemmli sample buffer containing phosphatase inhibitor cocktails 2 and 3. Phosphorylations of Akt (Ser473) is detected by western blotting. Platelet aggregation is measured as the optical density of PRP during aggregation using a platelet aggregometer, with the platelet-poor plasma 100% standard for change in optical density.

References

Selleck's Methyl-β-cyclodextrin (MβCD) has been cited by 14 publications

Extracellular Vesicle Spherical Nucleic Acids [ JACS Au, 2024, 4(6):2381-2392]	PubMed: 38938802
Real-Time Dissection of the Transportation and miRNA-Release Dynamics of Small Extracellular Vesicles [ Adv Sci (Weinh), 2023, 10(7):e2205566]	PubMed: 36599707
Exosomal miR-222-3p contributes to castration-resistant prostate cancer by activating mTOR signaling [ Cancer Sci, 2023, 114(11):4252-4269]	PubMed: 37671589
Exosomal miR-222-3p contributes to castration-resistant prostate cancer by activating mTOR signaling [ Cancer Sci, 2023, 114(11):4252-4269]	PubMed: 37671589
Porcine deltacoronavirus enters ST cells by clathrin-mediated endocytosis and does not require Rab5, Rab7, or Rab11 [ Microbiol Spectr, 2023, 10.1128/spectrum.02553-23]	PubMed: 37962380
Evaluation of the liver targeting and anti‑liver cancer activity of artesunate‑loaded and glycyrrhetinic acid‑coated nanoparticles [ Exp Ther Med, 2023, 26(5):516]	PubMed: 37854499
Evaluation of the liver targeting and anti‑liver cancer activity of artesunate‑loaded and glycyrrhetinic acid‑coated nanoparticles [ Exp Ther Med, 2023, 26(5):516]	PubMed: 37854499
CNPY4 inhibits the Hedgehog pathway by modulating membrane sterol lipids [ Nat Commun, 2022, 13(1):2407]	PubMed: 35504891
Channel catfish virus entry into host cells via clathrin-mediated endocytosis [ Virus Res, 2022, 315:198794]	PubMed: 35504448
Porcine Deltacoronavirus (PDCoV) Entry into PK-15 Cells by Caveolae-Mediated Endocytosis [ Viruses, 2022, 14(3)496]	PubMed: 35336903

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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