Melittin

Catalog No.S9794 Batch:S979401

Technical Data

Formula	C₁₃₁H₂₂₉N₃₉O₃₁
Molecular Weight	2846.46	CAS No.	20449-79-0, 37231-28-0
Solubility (25°C)*	In vitro	Water	100 mg/mL (35.13 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Melittin (MLT, Forapin, Forapine) is an activator of phospholipase A2 (PLA2) that stimulates the activity of the low molecular weight PLA2, while it does not the increase the activity of the high molecular weight enzyme.

Targets

PLA2 ^[1]

In vitro

In vitro studies using TNF-α/IFN-γ-stimulated human keratinocytes shows that melittin inhibits the increased expression of chemokines, such as CCL17 and CCL22, and pro-inflammatory cytokines, including IL-1β, IL-6 and IFN-γ, through the blockade of the NF‐κB and STAT signalling pathways.^[2]

In vivo

Melittin exhibits potent anti-atopic activities, shown by decreased Atopic dermatitis (AD)-like skin lesions, induced by DNCB in mice.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines HaCaT cell line Concentrations 0.1 μg/mL, 0.5 μg/mL, 1 μg/mL Incubation Time 30 min Method The human keratinocyte HaCaT cell line is cultured in high glucose DMEM supplemented with 10% (v/v) FBS and antibiotics at 37°C in a humidified 5% CO2 incubator. HaCaT (5.0 × 10⁵ cells/mL) cells are seeded in the complete medium. 24 hours later, the medium is changed to serumfree mediumwith bee venom (1, 10 and 100 ng/mL) and melittin (0.1, 0.5 and 1 μg/mL). After 30 min, the cells are stimulated with 10 ng/mL TNF-α/IFN-γ for 9 h.
Animal Study:^{^[2]}	Animal Models 6-week-old female Balb/c mice Dosages 100 μg, 200 μg, 500 μg Administration Topical

Cell Assay:^{^[2]}

Cell lines
HaCaT cell line
Concentrations
0.1 μg/mL, 0.5 μg/mL, 1 μg/mL
Incubation Time
30 min
Method
The human keratinocyte HaCaT cell line is cultured in high glucose DMEM supplemented with 10% (v/v) FBS and antibiotics at 37°C in a humidified 5% CO2 incubator. HaCaT (5.0 × 10⁵ cells/mL) cells are seeded in the complete medium. 24 hours later, the medium is changed to serumfree mediumwith bee venom (1, 10 and 100 ng/mL) and melittin (0.1, 0.5 and 1 μg/mL). After 30 min, the cells are stimulated with 10 ng/mL TNF-α/IFN-γ for 9 h.

Animal Study:^{^[2]}

Animal Models
6-week-old female Balb/c mice
Dosages
100 μg, 200 μg, 500 μg
Administration
Topical

References

Selleck's Melittin has been cited by 3 publications

Melittin promotes the proliferation of Schwann cells in hyperglycemic environment by up‑regulating the Crabp2/Wnt/β‑catenin signaling pathway [ Mol Med Rep, 2025, 31(1)5]	PubMed: 39450531
Melittin induces autophagy to alleviate chronic renal failure in 5/6-nephrectomized rats and angiotensin II-induced damage in podocytes [ Nutr Res Pract, 2024, 18(2):210-222]	PubMed: 38584815
PGE2 -EP3 axis promotes brown adipose tissue formation through stabilization of WTAP RNA methyltransferase [ EMBO J, 2022, e110439]	PubMed: 35781818

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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