Lucigenin

Catalog No.S6824 Batch:S682401

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Technical Data

Formula

C28H22N4O6
Molecular Weight 510.5 CAS No. 2315-97-1
Solubility (25°C)* In vitro DMSO 20 mg/mL (39.17 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Lucigenin (NSC-151912, L-6868) is a fluoerscent probe that exhibits a bluish-green fluorescence in the presence of endogenously generated superoxide anion radicals and chloride in cells.
Targets
superoxide anion radical [1] chloride [1]
In vitro

1.1 Preparation of the stock solution
Dissolve 1 mg of Lucigenin in 0.1919 mL of DMSO to obtain 10 mM of Lucigenin.
Note: It is recommended to store the stock solution at -20 °C -80 °C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Lucigenin working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of Lucigenin working solution.
Note: Please adjust the concentration of Lucigenin working solution according to the actual situation.
Cell staining
2.1 Cell preparation.
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of Lucigenin working solution, and then incubate at room temperature for 15 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope.

Protocol (from reference)

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.