Liproxstatin-1

Catalog No.S7699 Batch:S769906

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Technical Data

Formula

C19H21ClN4
Molecular Weight 340.85 CAS No. 950455-15-9
Solubility (25°C)* In vitro DMSO Insoluble
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Liproxstatin-1 is a potent ferroptosis inhibitor with an IC50 of 22 nM.
Targets
ferroptosis [1]
(Cell-free assay)
22 nM
In vitro Liproxstatin-1 is able to inhibit ferroptosis in the low nanomolar range and inhibit the growth of Gpx4−/−cells with IC50 of 22 nM. Liproxstatin-1 (50 nM) completely prevents lipid peroxidation in Gpx4−/−cells. Liproxstatin-1 (200 nM) protects against FINs, such as BSO (10 µM), erastin (1 µM) and RSL3 (0.5 µM), in a dose dependent manner, whereas it fails to rescue cell death induced by staurosporine (0.2 µM) and H2O2 (200 µM).[1]
In vivo Liproxstatin-1 remarkably extends survival compared with the vehicle-treated group, delays ferroptosis in tubular cells, and mitigates tissue injury in ischaemia/reperfusion-induced liver injury. [1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Gpx4−/−cells

  • Concentrations

    ~200 nM

  • Incubation Time

    ~72 h

  • Method

    Cell viability is assessed at different time points after treatment using AquaBluer according to the manufacturer’s recommendations. Alternatively, cell death is also quantified by measuring released lactate dehydrogenase activity using the cytotoxicity detection kit (LDH).
Animal Study:

[1]
  • Animal Models

    GreERT2; Gpx4fI/fI mice

  • Dosages

    10 mg/kg

  • Administration

    i.p.

Customer Product Validation

,

Data from [Data independently produced by , , Mol Psychiatry, 2017, 22(11):1520-1530]

Data from [Data independently produced by , , Cancer Res, 2017, 77(8):2064-2077]

Data from [Data independently produced by , , Redox Biol, 2017, 12:8-17]

Selleck's Liproxstatin-1 has been cited by 246 publications

7-Dehydrocholesterol dictates ferroptosis sensitivity [ Nature, 2024, 626(7998):411-418.] PubMed: 38297130
7-Dehydrocholesterol dictates ferroptosis sensitivity [ Nature, 2024, 626(7998):411-418.] PubMed: 38297130
CircPIAS1 promotes hepatocellular carcinoma progression by inhibiting ferroptosis via the miR-455-3p/NUPR1/FTH1 axis [ Mol Cancer, 2024, 23(1):113] PubMed: 38802795
Sensing steroid hormone 17α-hydroxypregnenolone by GPR56 enables protection from ferroptosis-induced liver injury [ Cell Metab, 2024, S1550-4131(24)00371-1] PubMed: 39389061
Ferroptosis-like cell death promotes and prolongs inflammation in Drosophila [ Nat Cell Biol, 2024, 10.1038/s41556-024-01450-7] PubMed: 38918597
PRDX6 augments selenium utilization to limit iron toxicity and ferroptosis [ Nat Struct Mol Biol, 2024, 10.1038/s41594-024-01329-z] PubMed: 38867112
Pro-ferroptotic signaling promotes arterial aging via vascular smooth muscle cell senescence [ Nat Commun, 2024, 15(1):1429] PubMed: 38365899
SLC13A3 is a major effector downstream of activated β-catenin in liver cancer pathogenesis [ Nat Commun, 2024, 15(1):7522] PubMed: 39215042
Fatal COVID-19 pulmonary disease involves ferroptosis [ Nat Commun, 2024, 15(1):3816] PubMed: 38769293
Inhibition of GPX4 enhances CDK4/6 inhibitor and endocrine therapy activity in breast cancer [ Nat Commun, 2024, 15(1):9550] PubMed: 39500869

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.