Liproxstatin-1

Catalog No.S7699 Batch:S769905

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Technical Data

Formula

C19H21ClN4
Molecular Weight 340.85 CAS No. 950455-15-9
Solubility (25°C)* In vitro DMSO 68 mg/mL (199.5 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Liproxstatin-1 is a potent ferroptosis inhibitor with an IC50 of 22 nM.
Targets
ferroptosis [1]
(Cell-free assay)
22 nM
In vitro Liproxstatin-1 is able to inhibit ferroptosis in the low nanomolar range and inhibit the growth of Gpx4−/−cells with IC50 of 22 nM. Liproxstatin-1 (50 nM) completely prevents lipid peroxidation in Gpx4−/−cells. Liproxstatin-1 (200 nM) protects against FINs, such as BSO (10 µM), erastin (1 µM) and RSL3 (0.5 µM), in a dose dependent manner, whereas it fails to rescue cell death induced by staurosporine (0.2 µM) and H2O2 (200 µM).[1]
In vivo Liproxstatin-1 remarkably extends survival compared with the vehicle-treated group, delays ferroptosis in tubular cells, and mitigates tissue injury in ischaemia/reperfusion-induced liver injury. [1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Gpx4−/−cells

  • Concentrations

    ~200 nM

  • Incubation Time

    ~72 h

  • Method

    Cell viability is assessed at different time points after treatment using AquaBluer according to the manufacturer’s recommendations. Alternatively, cell death is also quantified by measuring released lactate dehydrogenase activity using the cytotoxicity detection kit (LDH).
Animal Study:

[1]
  • Animal Models

    GreERT2; Gpx4fI/fI mice

  • Dosages

    10 mg/kg

  • Administration

    i.p.

Customer Product Validation

,

Data from [Data independently produced by , , Mol Psychiatry, 2017, 22(11):1520-1530]

Data from [Data independently produced by , , Cancer Res, 2017, 77(8):2064-2077]

Data from [Data independently produced by , , Redox Biol, 2017, 12:8-17]

Selleck's Liproxstatin-1 has been cited by 262 publications

S100P is a ferroptosis suppressor to facilitate hepatocellular carcinoma development by rewiring lipid metabolism [ Nat Commun, 2025, 16(1):509] PubMed: 39779666
A transcriptional atlas of gut-innervating neurons reveals activation of interferon signaling and ferroptosis during intestinal inflammation [ Neuron, 2025, S0896-6273(25)00136-9] PubMed: 40101721
Inhibition of Endoplasmic Reticulum Stress Cooperates with SLC7A11 to Promote Disulfidptosis and Suppress Tumor Growth upon Glucose Limitation [ Adv Sci (Weinh), 2025, 12(7):e2408789] PubMed: 39739602
SLC25A1 and ACLY maintain cytosolic acetyl-CoA and regulate ferroptosis susceptibility via FSP1 acetylation [ EMBO J, 2025, 10.1038/s44318-025-00369-5] PubMed: 39881208
Upregulated FSP1 by GPD1/1L mediated lipid droplet accumulation enhances ferroptosis resistance and peritoneal metastasis in gastric cancer [ Cell Commun Signal, 2025, 23(1):132] PubMed: 40075460
Targeting oxidative stress-induced lipid peroxidation enhances podocyte function in cystinosis [ J Transl Med, 2025, 23(1):206] PubMed: 39980044
Lycium barbarum glycopeptide reduces brain damage following ischemic stroke by inhibiting ferroptosis and oxidation [ Neural Regen Res, 2025, 10.4103/NRR.NRR-D-24-00747] PubMed: 39930986
Exogenous dihomo-γ-linolenic acid triggers ferroptosis via ACSL4-mediated lipid metabolic reprogramming in acute myeloid leukemia cells [ Transl Oncol, 2025, 52:102227] PubMed: 39644823
Inhibition of Hippocampal Neuronal Ferroptosis by Liproxstatin-1 Improves Learning and Memory Function in Aged Mice with Perioperative Neurocognitive Dysfunction [ J Inflamm Res, 2025, 18:2991-3007] PubMed: 40046685
Involvement of STAT3 activation in ameliorating all-trans-retinal-induced ferroptosis in photoreceptor-derived 661W cells in vitro [ Exp Eye Res, 2025, 253:110280] PubMed: 39947311

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.