Levothyroxine (L-Thyroxine) sodium

Catalog No.S3747 Batch:S374703

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Technical Data

Formula

C15H10I4NO4.Na
Molecular Weight 798.85 CAS No. 55-03-8
Solubility (25°C)* In vitro DMSO 100 mg/mL (125.17 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Levothyroxine (L-Thyroxine) sodium is the major hormone derived from the thyroid gland. It is the agonist of Thyroid hormone receptor alpha and beta.
In vitro Levothyroxine can upregulate the expression of P-gp mRNA and protein in LS180 cells and can produce the same effect in Caco-2 cells, which are relatively lacking in PXR. It doea not affect the expression of CYP3A4 in either cell line[1].
In vivo Levothyroxine is a synthetic T4 hormone that is biochemically and physiologically indistinguishable from the natural one, and it is administered when the body is deficient in the natural hormone. Its nain site of absorption is small intestine, more specifically through the duodenum, jejunum and ileum. Very little is absorbed in the stomach. The bioavailability is about 70-80 % in euthyroid person and may be slightly higher in hyperthyroid patients. The absorption of levothyroxine appears to be influenced by gastric pH. Tmax=2-3 hours and the half-time T1/2 is 6.2 and 7.5 days in euthyroid and hypothyroid patients, respectively[3]. T4 hormones is known to modulate the expression of ionic channels, pumps and regulatory contractile proteins. T4-treatment induces a dose-dependent increase in the duration of contractions. Abnormal uterine contractile pattern are induced by T4-treatment[2].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    LS180V cells

  • Concentrations

    100 nM and 100 μM

  • Incubation Time

    72 h

  • Method

    LS180V cells are induced for 72 h with two different concentrations of l-T4: 100 nM and 100 μM.

Animal Study:[2]
  • Animal Models

    Sprague-Dawley rats

  • Dosages

    20 μg/kg/day and 100 μg/kg/day

  • Administration

    i.p.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.