Levofloxacin hydrate

Catalog No.S4604 Batch:S460401

Technical Data

Formula	C₁₈H₂₀FN₃O₄.1/2H₂O
Molecular Weight	370.38	CAS No.	138199-71-0
Solubility (25°C)*	In vitro	DMSO	74 mg/mL (199.79 mM)
		Ethanol	10 mg/mL (26.99 mM)
		Water	5 mg/mL (13.49 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Levofloxacin (Levaquin, Tavanic, Quixin, Iquix, Cravit) is a broad-spectrum, third-generation fluoroquinolone antibiotic and optically active L-isomer of ofloxacin with antibacterial activity. It acts by inhibiting DNA gyrase (bacterial topoisomerase II).

Targets

Topoisomerase II ^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines skin mast cells(from dogs and rats) Concentrations 30 to 1000 mg/mL Incubation Time 30 min Method Cell suspension samples (50 mL) (2-5×10⁴ mast cells in each tube for dispersed skin cells or 1 ×10⁵ mast cells in each tube for rat peritoneal cells) in duplicate are equilibrated at 37℃ for 10 min, and then added to polypropylene tubes containing 450 mL test compounds dissolved in Tyrode solution. Histamine release reaction is allowed to proceed for a further 30 min. at the end of which time the reaction is terminated by adding 2 mL ice-cold Tyrode solution and the mixture is centrifuged (450 g for 5 min at 4℃). For determination of total histamine content, mast cells are disrupted in boiling water for 10 min. Histamine concentrations in the resulting supernatant and disrupted cells are determined by the enzyme immunoassay described above.
Animal Study:^{^[1]}	Animal Models Adult beagle dogs, male Sprague-Dawley rats Dosages 0 3, 1, 3 and 10 mg/kg for dogs and 10, 30 and 100 mg/kg for rats Administration i.v.

Cell Assay:^{^[1]}

Cell lines
skin mast cells(from dogs and rats)
Concentrations
30 to 1000 mg/mL
Incubation Time
30 min
Method
Cell suspension samples (50 mL) (2-5×10⁴ mast cells in each tube for dispersed skin cells or 1 ×10⁵ mast cells in each tube for rat peritoneal cells) in duplicate are equilibrated at 37℃ for 10 min, and then added to polypropylene tubes containing 450 mL test compounds dissolved in Tyrode solution. Histamine release reaction is allowed to proceed for a further 30 min. at the end of which time the reaction is terminated by adding 2 mL ice-cold Tyrode solution and the mixture is centrifuged (450 g for 5 min at 4℃). For determination of total histamine content, mast cells are disrupted in boiling water for 10 min. Histamine concentrations in the resulting supernatant and disrupted cells are determined by the enzyme immunoassay described above.

Animal Study:^{^[1]}

Animal Models
Adult beagle dogs, male Sprague-Dawley rats
Dosages
0 3, 1, 3 and 10 mg/kg for dogs and 10, 30 and 100 mg/kg for rats
Administration
i.v.

References

[1] Mori K, et al. J Pharm Pharmacol. 2000, 52(5):577-584.

Selleck's Levofloxacin hydrate has been cited by 3 publications

Nano-in-Microparticles for Aerosol Delivery of Antibiotic-Loaded, Fucose-Derivatized, and Macrophage-Targeted Liposomes to Combat Mycobacterial Infections: In Vitro Deposition, Pulmonary Barrier Interactions, and Targeted Delivery [ Adv Healthc Mater, 2022, e2102117]	PubMed: 35112802
Effect of intravitreal conbercept injection on VEGF-A and -B levels in the aqueous and vitreous humor of patients with proliferative diabetic retinopathy [ Exp Ther Med, 2021, 21(4):332]	PubMed: 33732305
Identification of Small-Molecule Inhibitors of Brucella Diaminopimelate Decarboxylase by Using a High-Throughput Screening Assay. [ Front Microbiol, 2020, 10:2936]	PubMed: 32038511

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.