Leronlimab (anti-CCR5)

Catalog No.A2040        Batch: A204001

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Technical Data

CAS No. 674782-26-4
Formulation 100 mM Pro-Ac, 20 mM Arg, pH5.0
Isotype Human IgG4
Application Leronlimab (anti-CCR5)
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles.
Purity 99%
Protein concentration 1.94mg/ml
Endotoxin Level <1 EU/mg

Biological Activity

Description Leronlimab (anti-CCR5) （PRO 140; Vyrologix）is a humanized monoclonal antibody to CCR5, blocks breast cancer cellular metastasis and enhances cell death induced by DNA damaging chemotherapy. It is being investigated as a potential therapy in the treatment of HIV infection.
In vitro

Leronlimab binds CCR5 in multiple breast cancer cell lines, reducing ligand-induced Ca+ 2 signaling, invasion of TNBC into Matrigel, and transwell migration, also enhances the breast cancer (BCa) cell killing of the BCa chemotherapy reagent, doxorubicin.[1]
In vivo

In xenografts conducted with Nu/Nu mice, leronlimab reduces lung metastasis of the TNBC cell line, MB-MDA-231, reducing the metastatic tumor burden of established TNBC lung metastasis.[1]

Protocol (Only for Reference)

Cell Assay:

  • Equal numbers of cells were plated, and after 72 h of exposure to the drugs, cells were incubated with 1 mg/mL of MTT for 90 min. The reduced (insoluble and colored) formazan was dissolved in 0.04 N HCl acidified isopropanol and measured spectrophotometrically at 570 nm.
Animal Study:

  • AnimalModels:Female NCI Athymic nu/nu nude mice injected with MB-MDA-231 cells expressing Luc2-eGFP
    Dosages:2 mg/mouse
    Administration:i.p.[1]

     

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

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