LDN-212854

Catalog No.S7147 Batch:S714701

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Technical Data

Formula

C25 H22 N6
Molecular Weight 406.48 CAS No. 1432597-26-6
Solubility (25°C)* In vitro DMSO 81 mg/mL (199.27 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
0.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
0.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description LDN-212854 (BMP Inhibitor III) is a potent and selective BMP receptor inhibitor with IC50 of 1.3 nM for ALK2, about 2-, 66-, 1641-, and 7135-fold selectivity over ALK1, ALK3, ALK4, and ALK5, respectively.
Targets
ALK2 [1]
(Cell-free assay)		 ALK1 [1]
(Cell-free assay)		 ALK3 [1]
(Cell-free assay)		 ALK4 [1]
(Cell-free assay)		 ALK5 [1]
(Cell-free assay)
1.3 nM 2.4 nM 85.8 nM 2133 nM 9276 nM
In vitro In myofibroblast C2C12 cells, LDN-212854 exhibits greater selectivity for BMP6- versus BMP4-induced osteogenic differentiation. [1]
In vivo LDN-212854 (6 mg/kg, i.p.) effectively neutralize ALK2 signaling in vivo, and potently inhibits heterotopic ossification in an inducible transgenic mutant ALK2 mouse model of fibrodysplasia ossificans progressiva. [1]

Protocol (from reference)

Animal Study:

[1]
  • Animal Models

    Murine inducible transgenic ALK2Q207D model of heterotopic ossification.

  • Dosages

    6 mg/kg, twice daily

  • Administration

    i.p.

Selleck's LDN-212854 has been cited by 8 publications

ADAMTS2 promotes radial migration by activating TGF-β signaling in the developing neocortex [ EMBO Rep, 2024, 25(7):3090-3115] PubMed: 38871984
Transcriptional Profiling Supports the Notochordal Origin of Chordoma and Its Dependence on a TGFΒ1-TBXT Network [ Am J Pathol, 2023, S0002-9440(23)00047-0] PubMed: 36804377
Transcriptional Profiling Supports the Notochordal Origin of Chordoma and Its Dependence on a TGFB1-TBXT Network [ Am J Pathol, 2023, 193(5):532-547] PubMed: 36804377
BMP9-ID1 Signaling Activates HIF-1α and VEGFA Expression to Promote Tumor Angiogenesis in Hepatocellular Carcinoma [ Int J Mol Sci, 2022, 23(3)1475] PubMed: 35163396
Clonal hematopoiesis with JAK2V617F promotes pulmonary hypertension with ALK1 upregulation in lung neutrophils [ Nat Commun, 2021, 12(1):6177] PubMed: 34702814
BMP9-ID1 signaling promotes EpCAM-positive cancer stem cell properties in hepatocellular carcinoma [ Mol Oncol, 2021, 10.1002/1878-0261.12963] PubMed: 33834612
KR‑12‑a6 promotes the osteogenic differentiation of human bone marrow mesenchymal stem cells via BMP/SMAD signaling. [ Mol Med Rep, 2020, 21(1):61-68] PubMed: 31939626
ALK1 Loss Results in Vascular Hyperplasia in Mice and Humans Through PI3K Activation. [ Arterioscler Thromb Vasc Biol, 2018, 38(5):1216-1229] PubMed: 29449337

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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