L-SelenoMethionine

Catalog No.S3973 Batch:S397302

Technical Data

Formula	C₅H₁₁NO₂Se
Molecular Weight	196.11	CAS No.	3211-76-5
Solubility (25°C)*	In vitro	Water	39 mg/mL (198.86 mM)
		DMSO	Insoluble

* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	L-SelenoMethionine (SeMet), a naturally occurring amino acid, is a major food-form of selenium. It has been used as a supplement, and exhibits some important functions like cancer prevention and antioxidative defense.
In vitro	L-SelenoMethionine (SeMet) has also been shown to contribute to the mitigation of radiation-induced oxidative stress. L-SelenoMethionine has the ability to selectively induce apoptosis and growth arrest in cancerous, but not normal, human prostate cells. Mechanistically, SeMet treatment is shown to promote growth inhibition in human colon cancer cells through the phosphorylation of histone H3 via the activation of MAPK extracellular regulated kinase (ERK) and subsequent phosphorylation of the ribosomal S6 kinase (RSK)^[1].
In vivo	L-SelenoMethionine (SeMet) increases survival time of NK/Ly lymphoma bearing animals treated with doxorubicin. L-SelenoMethionine (SeMet) can effectively protect the hematopoietic system and liver of tumor-bearing animals with NK/Ly lymphoma from the side effects of anticancer drug doxorubicin, which is revealed by normalization of blood indexes, animal weight, and ALT/AST activity^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Human thyroid epithelial cells (HTori-3) Concentrations 5 μM Incubation Time 24 h Method Human thyroid epithelial cells (HTori-3) are maintained in Dulbecco's modified Eagle's medium (DMEM)/F12 supplemented with 1% glutamine and 10% FBS (growth medium). Twenty-four hours prior to irradiation with iron ions, fresh medium with or without 5 μM SeM is added. At the time of radiation exposure, the cells are approximately 80% confluent.
Animal Study:^{^[2]}	Animal Models NL/Ly lymphoma-bearing mice Dosages 60 μg/kg, cumulative dose 600 μg/kg Administration p.o.

Cell Assay:^{^[1]}

Cell lines
Human thyroid epithelial cells (HTori-3)
Concentrations
5 μM
Incubation Time
24 h
Method
Human thyroid epithelial cells (HTori-3) are maintained in Dulbecco's modified Eagle's medium (DMEM)/F12 supplemented with 1% glutamine and 10% FBS (growth medium). Twenty-four hours prior to irradiation with iron ions, fresh medium with or without 5 μM SeM is added. At the time of radiation exposure, the cells are approximately 80% confluent.

Animal Study:^{^[2]}

Animal Models
NL/Ly lymphoma-bearing mice
Dosages
60 μg/kg, cumulative dose 600 μg/kg
Administration
p.o.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.