L-Citrulline

Catalog No.S3798 Batch:S379801

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Technical Data

Formula

C6H13N3O3
Molecular Weight 175.19 CAS No. 372-75-8
Solubility (25°C)* In vitro Water 35 mg/mL (199.78 mM)
DMSO Insoluble
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description L-citrulline is a substance called a non-essential amino acid that is used as a sports performance and cardiovascular health supplement.
In vitro L-Citrulline restores nitric oxide/reactive oxygen species balance and barrier function in high glucose-treated monolayers of human glomerular endothelial cells. Moreover, L-Citrulline also has the ability to establish an anti-inflammatory profile, characterized by increased IL-10 and reduced IL-1β and IL-12(p70) generation in the human proximal tubular cells[1].
In vivo Oral l-cit (precursor of l-arg) augments NO-dependent signaling, not only by means of increasing l-arg synthesis, but also by decreasing l-arg catabolism, as such increasing circulating l-arg levels. L-Citrulline exerts protective effects in kidneys of STZ-rats, and markedly reduces urinary albumin excretion, tubulo-interstitial fibrosis, and kidney hypertrophy, observed in untreated diabetic mice[1]. L-citrulline supplementation also strengthens vascular endothelium function and attenuates stress-induced cold hypersensitivity by improving blood circulation[2].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    Human glomerular endothelial cells

  • Concentrations

    1 mM

  • Incubation Time

    2 h

  • Method

    Human glomerular endothelial cell are seeded at 1 × 105 cells per well in 24-well plates. Confluent quiescent cell monolayers are exposed to HG or proper iso-osmotic control for 24 h. L-Citrulline (1 mM) is applied 2 h prior to HG. Exposure is terminated by removal of the supernatant. Fresh basic CSC medium is replaced and cells incubated for additional 30 min. Supernatant is then removed, subsequently centrifuged and stored at −80°C for NO analysis.
Animal Study:[1]
  • Animal Models

    diabetic mice (C57BL/6 mice, intraperitoneal injections of STZ)

  • Dosages

    50 mg/kg

  • Administration

    oral

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.