Kobe2602

Catalog No.S6527 Batch:S652702

Technical Data

Formula	C₁₄H₉F₄N₅O₄S
Molecular Weight	419.31	CAS No.	454453-49-7
Solubility (25°C)*	In vitro	DMSO	84 mg/mL (200.32 mM)
		Ethanol	14 mg/mL (33.38 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Kobe2602 is a selective Ras inhibitor that blocks H-Ras GTP binding to c-Raf-1 (Ki = 149 µM).

Targets

Ras ^[1]

149 μM(Ki)

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines NIH 3T3 cells Concentrations 20 μM Incubation Time 1 h Method NIH 3T3 cells are transfected with pEF-BOS-HA-K-Ras(G12V) or an empty vector and treated with 20 μM Kobe0065, 20 μM Kobe2602, or the vehicle (DMSO) in the presence of 2% (vol/vol) FBS for 1 h. Cell lysate is subjected to detection of c-Raf-1 coimmunoprecipitated with K-Ras(G12V) by an anti-HA antibody and total c-Raf-1 by Western blotting with an anti-c-Raf-1 antibody.
Animal Study:^{^[1]}	Animal Models Female athymic nude mice implanted with SW480 cells Dosages 80 mg/kg Administration oral

Cell Assay:^{^[1]}

Cell lines
NIH 3T3 cells
Concentrations
20 μM
Incubation Time
1 h
Method
NIH 3T3 cells are transfected with pEF-BOS-HA-K-Ras(G12V) or an empty vector and treated with 20 μM Kobe0065, 20 μM Kobe2602, or the vehicle (DMSO) in the presence of 2% (vol/vol) FBS for 1 h. Cell lysate is subjected to detection of c-Raf-1 coimmunoprecipitated with K-Ras(G12V) by an anti-HA antibody and total c-Raf-1 by Western blotting with an anti-c-Raf-1 antibody.

Animal Study:^{^[1]}

Animal Models
Female athymic nude mice implanted with SW480 cells
Dosages
80 mg/kg
Administration
oral

References

[1] Shima F, et al. Proc Natl Acad Sci U S A. 2013, 110(20):8182-7.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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