JHU-083

Catalog No.S8891 Batch:S889101

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Technical Data

Formula

C14H24N4O4
Molecular Weight 312.36 CAS No. 1998725-11-3
Solubility (25°C)* In vitro DMSO 62 mg/mL (198.48 mM)
Ethanol 62 mg/mL (198.48 mM)
Water (warmed with 50ºC water bath) 3 mg/mL (9.6 mM)
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
3.1mg/ml Taking the 1 mL working solution as an example, add 50 μL 62 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description JHU-083 (Ethyl 2-(2-Amino-4-methylpentanamido)-DON) is a novel prodrug of DON.JHU-083 selectively blocks glutaminase activity in brain CD11b+ cells and prevents depression-associated behaviors induced by chronic social defeat stress.Solutions are unstable and should be fresh-prepared.
Targets
Glutaminase [1]
In vitro

JHU-083 selectively inhibits T-cell proliferation and decreases T-cell activation, with no effect on DCs.[2]
In vivo

In vivo, orally administered JHU-083 significantly decreases the experimental autoimmune encephalomyelitis(EAE) severity in both prevention and treatment paradigms and reverses EAE-induced cognitive impairment.[2]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    CD4+ T cells

  • Concentrations

    1 μM, 3 μM, 10 μM, 30 μM, or 100 μM

  • Incubation Time

    72h

  • Method

    150,000 CD4+ T cells are plated with 20 μM eFlur450 cell proliferation dye, 2 μg/mL α-CD28, and ±JHU-083 in 100 μM complete RPMI. Every 24 hours, cells are treated with 20 μM media ±JHU-083.
Animal Study:

[2]
  • Animal Models

    C57BL/6J mice

  • Dosages

    1.83mg/kg

  • Administration

    oral

Selleck's JHU-083 has been cited by 1 publication

Mitochondrial transplantation ameliorates doxorubicin-induced cardiac dysfunction via activating glutamine metabolism [ iScience, 2023, 26(10):107790] PubMed: 37731615

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.