JC-1

Catalog No.S9784 Batch:S978401

Technical Data

Formula	C₂₅H₂₇Cl₅N₄
Molecular Weight	560.77	CAS No.	3520-43-2
Solubility (25°C)*	In vitro	DMSO	33 mg/mL (58.84 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	JC-1 (CBIC2, NK 1420), a fluorescent lipophilic carbocyanine dye, is a mitochondrial potential (ΔΨ(m)) marker. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers.
In vitro	1. Take the 6-well plate as an example for cell planking, and the density is 5×10^5/mL. Incubate overnight in 5% CO2 incubator at 37℃. Note: it is suggested that the cell density during apoptosis induction should not exceed 1×10^6/ml, which can also be cultured to the appropriate density according to your own cell type. 2. Take 0.5 mL suspension into sterile centrifuge tube; 400 g centrifugation for 3-5 min; Discard the supernatant. 3. The cells were resuspended with 1mljc-1 working solution and incubated in 5% CO2 incubator at 37℃for 15-30 min. 4. Centrifugation at room temperature for 5 min at 400 g; Suck of the supernatant. 5. The cells were resuspended with 2 mL cell culture medium or buffer, and then centrifuged at room temperature for 5 min at 400 g; Discard the supernatant and repeat twice. 6. Resuspend the cells with 1mL of fresh culture medium or buffer, and immediately conduct subsequent flow cytometry or fluorescence microscope observation. 7. Data analysis (flow cytometry) : mitochondria of healthy cells containing red JC-1 aggregates were detected by FL2 channel; Apoptotic or unhealthy cells containing green JC-1 monomer were detected by FL1 (FITC) channel. 8. If used for enzyme labeling instrument, use 300 μL buffer resuspended cells; Then 100 per hole μ Transfer the stained cells to a light tight 96 well plate with the amount of L, and then conduct fluorescent enzyme label plate analysis.

Description

JC-1 (CBIC2, NK 1420), a fluorescent lipophilic carbocyanine dye, is a mitochondrial potential (ΔΨ(m)) marker. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers.

In vitro

1. Take the 6-well plate as an example for cell planking, and the density is 5×10^5/mL. Incubate overnight in 5% CO2 incubator at 37℃.
Note: it is suggested that the cell density during apoptosis induction should not exceed 1×10^6/ml, which can also be cultured to the appropriate density according to your own cell type.
2. Take 0.5 mL suspension into sterile centrifuge tube; 400 g centrifugation for 3-5 min; Discard the supernatant.
3. The cells were resuspended with 1mljc-1 working solution and incubated in 5% CO2 incubator at 37℃for 15-30 min.
4. Centrifugation at room temperature for 5 min at 400 g; Suck of the supernatant.
5. The cells were resuspended with 2 mL cell culture medium or buffer, and then centrifuged at room temperature for 5 min at 400 g; Discard the supernatant and repeat twice.
6. Resuspend the cells with 1mL of fresh culture medium or buffer, and immediately conduct subsequent flow cytometry or fluorescence microscope observation.
7. Data analysis (flow cytometry) : mitochondria of healthy cells containing red JC-1 aggregates were detected by FL2 channel; Apoptotic or unhealthy cells containing green JC-1 monomer were detected by FL1 (FITC) channel.
8. If used for enzyme labeling instrument, use 300 μL buffer resuspended cells; Then 100 per hole μ Transfer the stained cells to a light tight 96 well plate with the amount of L, and then conduct fluorescent enzyme label plate analysis.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines hippocampal neurons and glial cells Concentrations 1 μg/ml Incubation Time 15 min Method JC-1 is dissolved in dimethylsulfoxide (DMSO) as a 2 mg/ml stock. JC-1 is excited at 490 nm and an optical image splitter device is attached to the microscope to separate spectrally the green and red components of JC-1 fluorescence.

References

Selleck's JC-1 has been cited by 2 publications

Irisin attenuates sepsis-induced cardiac dysfunction by attenuating inflammation-induced pyroptosis through a mitochondrial ubiquitin ligase-dependent mechanism [ Biomed Pharmacother, 2022, 152:113199]	PubMed: 35653888
Combining an Aurora Kinase Inhibitor and a Death Receptor Ligand/Agonist Antibody Triggers Apoptosis in Melanoma Cells and Prevents Tumor Growth in Preclinical Mouse Models [ Clin Cancer Res, 2015, 21(23):5338-48]	PubMed: 26152738

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.