Jaceosidin

Catalog No.S0931 Batch:S093101

Print

Technical Data

Formula

C17H14O7
Molecular Weight 330.29 CAS No. 18085-97-7
Solubility (25°C)* In vitro DMSO 66 mg/mL (199.82 mM)
Ethanol 6 mg/mL (18.16 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Jaceosidin, a flavonoid isolated from Artemisia vestita, possesses anti-tumor and anti-proliferative activities in many cancer cells. Jaceosidin induces apoptosis, activates Bax and down-regulates Mcl-1 and c-FLIP expression. Jaceosidin inhibits COX-2 expression and NF-κB activation.
Targets
Bax [1] Mcl-1 [1] c-FLIP [1] COX-2 [2] NF-κB [2]
In vitro

Jaceosidin induces apoptosis through Bax activation and downregulation of Mcl-1 and c-FLIP expression in human renal carcinoma Caki cells.[1]
In vivo

Jaceosidin blocks carrageenan-induced increase in leukocyte number and protein levels in air pouch exudates, inhibits COX-2 expression and NF-kappaB activation, and markedly reduces TNF-alpha, IL-1beta, and prostaglandin E2 (PGE(2)) levels, also inhibits hind paw edema induced by carrageenan.[2]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Human renal carcinoma (Caki, ACHN, A498, and 786-O cell lines)

  • Concentrations

    --

  • Incubation Time

    --

  • Method

    Caki cells were treated with 75 µM jaceosidin for 3 h. The mitochondrial membrane potential (MMP) was measured using a flow cytometer. Caki cells were treated with 75 µM jaceosidin for 12 and 24 h. Cytoplasmic fractions were analyzed for cytochrome c release. Caki cells were treated 75 µM jaceosidin for 1, 3, 6 h and stained for active Bax using conformation-specific antibodies. The fluorescence intensity was detected by flow cytometry. For the Bax oligomerization assay, Caki cells were treated with 75 µM jaceosidin for 1, 3, 6 h. After treatment, Bax monomers and oligomers were detected by western blotting. 
Animal Study:

[2]
  • Animal Models

    mice of air pouch model of inflammation, rats of paw edema model of inflammation

  • Dosages

    10, 20 mg/kg

  • Administration

    p.o., s.c.

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.