IWP-2

Catalog No.S7085 Batch:S708505

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Technical Data

Formula

C22H18N4O2S3
Molecular Weight 466.6 CAS No. 686770-61-6
Solubility (25°C)* In vitro DMF 20 mg/mL warmed with 50ºC water bath (42.86 mM)
DMSO 3 mg/mL warmed with 50ºC water bath (6.42 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description IWP-2 is an inhibitor of Wnt processing and secretion with IC50 of 27 nM in a cell-free assay, selective blockage of Porcn-mediated Wnt palmitoylation, does not affect Wnt/β-catenin in general and displays no effect against Wnt-stimulated cellular responses. IWP-2 specifically inhibits CK1δ.
Targets
Wnt [1]
(Cell-free assay)		 M82FCK1δ [3]
(Cell-free assay)
27 nM 40 nM
In vitro

IWP-2 is useful in both regenerative medicine and anticancer efforts. IWP-2 inactivates Porcn, a membrane-bound O-acyltransferase (MBOAT), and selectively inhibits palmitoylation of Wnt. IWP-2 blocks Wnt-dependent phosphorylation of Lrp6 receptor and Dvl2, and β-catenin accumulation. [1]
Features Selective inhibitor of Porcn-mediated Wnt secretion.

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    L-Wnt-STF cells

  • Concentrations

    5 μM

  • Incubation Time

    24 h

  • Method

    L-Wnt-STF cells that exhibit constitutive Wnt pathway activation were incubated with IWR (10μM) and IWP (5μM) compounds for 24 hrs prior to lysis. Cellular lysates were subjected to Western blot analysis.
Animal Study:

[2]
  • Animal Models

    Crl:CD1 (ICR) and FVB/N female mice

  • Dosages

    50 µg

  • Administration

    s.c.

Customer Product Validation

Data from [Data independently produced by , , Sci Rep, 2016, 6:32770.]

Data from [Data independently produced by , , Methods, 2016, 101:103-12.]

Data from [Data independently produced by , , Biochem Biophys Res Commun, 2017, 486(1):49-56]

Selleck's IWP-2 has been cited by 116 publications

A primate-specific endogenous retroviral envelope protein sequesters SFRP2 to regulate human cardiomyocyte development [ Cell Stem Cell, 2024, S1934-5909(24)00256-X] PubMed: 39146934
In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells [ Nat Commun, 2024, 15(1):1351] PubMed: 38355589
Derivation of human primordial germ cell-like cells in an embryonic-like culture [ Nat Commun, 2024, 15(1):167] PubMed: 38167821
Bi-allelic NRXN1α deletion in microglia derived from iPSC of an autistic patient increases interleukin-6 production and impairs supporting function on neuronal networking [ Brain Behav Immun, 2024, 123:28-42] PubMed: 39243986
Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition [ Dev Cell, 2024, S1534-5807(24)00297-1] PubMed: 38776925
Basal delamination during mouse gastrulation primes pluripotent cells for differentiation [ Dev Cell, 2024, S1534-5807(24)00176-X] PubMed: 38579720
Biosafe cerium oxide nanozymes protect human pluripotent stem cells and cardiomyocytes from oxidative stress [ J Nanobiotechnology, 2024, 22(1):132] PubMed: 38532378
Modelling myocardial ischemia/reperfusion injury with inflammatory response in human ventricular cardiac organoids [ Cell Prolif, 2024, e13762.] PubMed: 39377453
Extraembryonic mesoderm cells derived from human embryonic stem cells rely on Wnt pathway activation [ Cell Prolif, 2024, 10.1111/cpr.13761] PubMed: 39385268
Fibrotic extracellular matrix impacts cardiomyocyte phenotype and function in an iPSC-derived isogenic model of cardiac fibrosis [ Transl Res, 2024, 273:58-77] PubMed: 39025226

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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