IWP-2

Catalog No.S7085 Batch:S708502

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Technical Data

Formula

C22H18N4O2S3
Molecular Weight 466.6 CAS No. 686770-61-6
Solubility (25°C)* In vitro DMSO 2.33 mg/mL (4.99 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description IWP-2 is an inhibitor of Wnt processing and secretion with IC50 of 27 nM in a cell-free assay, selective blockage of Porcn-mediated Wnt palmitoylation, does not affect Wnt/β-catenin in general and displays no effect against Wnt-stimulated cellular responses. IWP-2 specifically inhibits CK1δ.
Targets
Wnt [1]
(Cell-free assay)		 M82FCK1δ [3]
(Cell-free assay)
27 nM 40 nM
In vitro

IWP-2 is useful in both regenerative medicine and anticancer efforts. IWP-2 inactivates Porcn, a membrane-bound O-acyltransferase (MBOAT), and selectively inhibits palmitoylation of Wnt. IWP-2 blocks Wnt-dependent phosphorylation of Lrp6 receptor and Dvl2, and β-catenin accumulation. [1]
Features Selective inhibitor of Porcn-mediated Wnt secretion.

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    L-Wnt-STF cells

  • Concentrations

    5 μM

  • Incubation Time

    24 h

  • Method

    L-Wnt-STF cells that exhibit constitutive Wnt pathway activation were incubated with IWR (10μM) and IWP (5μM) compounds for 24 hrs prior to lysis. Cellular lysates were subjected to Western blot analysis.
Animal Study:

[2]
  • Animal Models

    Crl:CD1 (ICR) and FVB/N female mice

  • Dosages

    50 µg

  • Administration

    s.c.

Customer Product Validation

Data from [Data independently produced by , , Sci Rep, 2016, 6:32770.]

Data from [Data independently produced by , , Methods, 2016, 101:103-12.]

Data from [Data independently produced by , , Biochem Biophys Res Commun, 2017, 486(1):49-56]

Selleck's IWP-2 has been cited by 110 publications

In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells [ Nat Commun, 2024, 15(1):1351] PubMed: 38355589
Derivation of human primordial germ cell-like cells in an embryonic-like culture [ Nat Commun, 2024, 15(1):167] PubMed: 38167821
Basal delamination during mouse gastrulation primes pluripotent cells for differentiation [ Dev Cell, 2024, S1534-5807(24)00176-X] PubMed: 38579720
Biosafe cerium oxide nanozymes protect human pluripotent stem cells and cardiomyocytes from oxidative stress [ J Nanobiotechnology, 2024, 22(1):132] PubMed: 38532378
iPSC-based modeling of preeclampsia identifies epigenetic defects in extravillous trophoblast differentiation [ iScience, 2024, 27(4):109569] PubMed: 38623329
Induced pluripotent stem cell (iPSC) modeling validates reduced GBE1 enzyme activity due to a novel variant, p.Ile694Asn, found in a patient with suspected glycogen storage disease IV [ Mol Genet Metab Rep, 2024, 39:101069] PubMed: 38516405
Engineered Cardiac Microtissue Biomanufacturing Using Human Induced Pluripotent Stem Cell Derived Epicardial Cells [ bioRxiv, 2024, 2024.05.13.593960] PubMed: 38798424
Dissecting peri-implantation development using cultured human embryos and embryo-like assembloids [ Cell Res, 2023, 10.1038/s41422-023-00846-8] PubMed: 37460804
Inhibition of Wnt activity improves peri-implantation development of somatic cell nuclear transfer embryos [ Natl Sci Rev, 2023, 10(9):nwad173] PubMed: 37593113
Inhibition of Wnt activity improves peri-implantation development of somatic cell nuclear transfer embryos [ Natl Sci Rev, 2023, 10(9):nwad173] PubMed: 37593113

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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