ITSA-1 (ITSA1)

Catalog No.S8323 Batch:S832301

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Technical Data

Formula

C13H7Cl2N3O

Molecular Weight 292.12 CAS No. 200626-61-5
Solubility (25°C)* In vitro DMSO 58 mg/mL (198.54 mM)
Ethanol 5 mg/mL (17.11 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO Corn oil
3.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 60 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description ITSA-1 (ITSA1) is an HDAC activator via TSA suppression, but shows no activity towards other HDAC inhibitors.
Targets
HDAC [1]
(Cell-free assay)

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    A549 cells

  • Concentrations

    50 μM

  • Incubation Time

    17.5 h

  • Method

    A549 cells ( 75% confluent) in 10-cm dishes are treated with DMSO (0.1%), TSA (500 nM), or tubacin (2 μM) for 4.5 h, and then either DMSO (0.1%) or ITSA1 (50 μM) is added for an additional 17.5 h. Samples are rinsed in PBS, trypsinized, and fixed for 2 h in 70% ethanol 30% PBS (4°C). Cells then are washed once in PBS and stored overnight in PBS (4°C).

Selleck's ITSA-1 (ITSA1) has been cited by 4 publications

Trichostatin A relieves anxiety-and depression-like symptoms in APP/PS1 mice [ Front Pharmacol, 2024, 15:1333235] PubMed: 38572429
Tumour cells are sensitised to ferroptosis via RB1CC1-mediated transcriptional reprogramming [ Clin Transl Med, 2022, 12(2):e747] PubMed: 35220675
Trichostatin A ameliorates Alzheimer's disease-related pathology and cognitive deficits by increasing albumin expression and Aβ clearance in APP/PS1 mice [ Alzheimers Res Ther, 2021, 13(1):7] PubMed: 33397436
Epigenetic Reprogramming with Antisense Oligonucleotides Enhances the Effectiveness of Androgen Receptor Inhibition in Castration-Resistant Prostate Cancer [ Cancer Res, 2018, 78(20):5731-5740] PubMed: 30135193

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.