Isoquercitrin

Catalog No.S3842 Batch:S384201

Technical Data

Formula	C₂₁H₂₀O₁₂
Molecular Weight	464.38	CAS No.	482-35-9
Solubility (25°C)*	In vitro	DMSO	92 mg/mL (198.11 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Isoquercitrin (Hirsutrin, 3-Glucosylquercetin, Quercetin 3-o-glucopyranoside), a flavonoid compound with anticancer activity isolated from Bidens bipinnata L, is an inhibitor of Wnt/β-catenin that acts downstream of the β-catenin nuclear translocation.

Targets

Wnt/β-catenin ^[1]

In vitro

Isoquercitrin exerts inhibitory effect on Wnt/β-catenin signaling and no toxic effects on colon cancer cell lines. It supresses the proliferation of colon cancer cells but does not affect non-tumoral colon cells. Isoquercitrin inhibits cell migration in colon cancer cells, but has also no apparent effect on the non-tumor colon cells. Isoquercitrin inhibits the signaling downstream of β-catenin nuclear translocation as isoquercitrin reverses the effect of β-catenin S33A, which cannot be phosphorylated and avoids degradation^[1].

In vivo

Isoquercitrin treatment significantly affects Xenopus embryo development, most likely by disturbing axial patterning. Isoquercitrin affects canonical Wnt signaling downstream of β-Catenin nucleus translocation^[1]. In animals receiving isoquercitrin, eosinophil counts are lower in the bronchoalveolar lavage fluid (BALF), blood and lung pa-renchyma. Neutrophil counts in blood and IL-5 levels in lung homogenate are lower in isoquercitrin-treated mice. No alterations in mononuclear cell numbers are observed. Isoquercitrin also demonstrates anti-asthmatic activity by inhibiting of carbachol- and leukotriene D4-induced contraction in guinea pig airways^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HCT-116 cells Concentrations 75 and 150 μM Incubation Time 24 h Method HCT-116 cells are treated with flavonoid isoquercitrin for 24 h at concentrations of 75 and 150 μM. DMSO (used as a vehicle to solubilize the flavonoids) is added to control cultures at 1% v/v. To stimulate the Wnt pathway, cells are treated with Wnt3a-conditioned medium (CM) for 6 h at 25% of conditioned medium. Cells are co-treated with Wnt3a-CM and isoquercitrin for 6 h at 75 or 150 μM.
Animal Study:^{^[2]}	Animal Models a murine model of asthma (Female BALB/c mice) Dosages 15 mg/kg Administration by oral gavage

Cell Assay:^{^[1]}

Cell lines
HCT-116 cells
Concentrations
75 and 150 μM
Incubation Time
24 h
Method
HCT-116 cells are treated with flavonoid isoquercitrin for 24 h at concentrations of 75 and 150 μM. DMSO (used as a vehicle to solubilize the flavonoids) is added to control cultures at 1% v/v. To stimulate the Wnt pathway, cells are treated with Wnt3a-conditioned medium (CM) for 6 h at 25% of conditioned medium. Cells are co-treated with Wnt3a-CM and isoquercitrin for 6 h at 75 or 150 μM.

Animal Study:^{^[2]}

Animal Models
a murine model of asthma (Female BALB/c mice)
Dosages
15 mg/kg
Administration
by oral gavage

References

Selleck's Isoquercitrin has been cited by 5 publications

Single-cell transcriptomics reveal extracellular vesicles secretion with a cardiomyocyte proteostasis signature during pathological remodeling [ Commun Biol, 2023, 6(1):79]	PubMed: 36681760
Identification of the main flavonoids of Abelmoschus manihot (L.) medik and their metabolites in the treatment of diabetic nephropathy [ Front Pharmacol, 2023, 14:1290868]	PubMed: 38313075
Periostin promotes nucleus pulposus cells apoptosis by activating the Wnt/β-catenin signaling pathway [ FASEB J, 2022, 36(7):e22369]	PubMed: 35747912
Novel Insights into the Molecular Regulation of Ribonucleotide Reductase in Adrenocortical Carcinoma Treatment [ Cancers (Basel), 2021, 13(16)4200]	PubMed: 34439352
The β-catenin/YAP signaling axis is a key regulator of melanoma-associated fibroblasts [ Signal Transduct Target Ther, 2019, 4:63]	PubMed: 31885878

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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