Isopsoralen

Catalog No.S3892 Batch:S389201

Technical Data

Formula	C₁₁H₆O₃
Molecular Weight	186.16	CAS No.	523-50-2
Solubility (25°C)*	In vitro	DMSO	14 mg/mL (75.2 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Isopsoralen (Angelicin), also known as angelicin, is a constituent of roots and leaves of angelica with anti-inflammatory activity and regulates LPS-induced inflammation via inhibiting MAPK/NF-κB pathways. It also shows antiviral activity against gammaherpesviruses.
In vitro	Isopsoralen treatment stimulates the accumulation of cartilage nodules in a dose-dependent manner. Isopsoralen enhances the expressions of chondrogenic marker genes such as collagen II, collagen X, OCN, Smad4 and Sox9 in a time-dependent manner. Furthermore, isopsoralen induces the activation of extracellular signal-regulated kinase (ERK) and p38 MAP kinase, but not that of c-jun N-terminal kinase (JNK). Isopsoralen significantly enhances the protein expression of BMP-2 in a time-dependent manner and mediates a chondromodulating effect by BMP-2 or MAPK signaling pathways^[2].
In vivo	Isopsoralen has significant osteoprotective effect in female and male mice with sex hormone deprivation. After administration of isopsoralen for 8 weeks, Bone strength increases and trabecular bone microstructure improves^[1]. After intravenous administration to wistar rats, the elimination half-lives of isopsoralen is 5.35 h. The area under the curves of the tissues for isopsoralen decrease in the following order: kidney > lung > liver > heart > spleen > brain. After oral administration to Wistar rats, the elimination half-lives of isopsoralen is 5.56 h, and its bioavailability is 70.35%^[3].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines ATDC5 cells Concentrations 0-1 μM Incubation Time 24 h Method ATDC5 cells are seeded in 96-well plate at a density of 5×10³ cells/well and incubated overnight in media supplemented with 10% FBS followed by treatment with various concentrations of isopsoralen. After 24h of incubation, cells are washed with phosphate-buffered saline (PBS) and then treated with media containing 100 μg/ml MTT for 2 h at 37℃. The cells are then washed with PBS, and dissolved in 200 μl of DMSO. The resulting solubilized purple formazan is quantified by measuring the absorbance at a wavelength of 540 nm using a spectrophotometer.
Animal Study:^{^[1]}	Animal Models ICR mice Dosages 10 mg/kg and 20 mg/kg Administration intragastrically

Cell Assay:^{^[2]}

Cell lines
ATDC5 cells
Concentrations
0-1 μM
Incubation Time
24 h
Method
ATDC5 cells are seeded in 96-well plate at a density of 5×10³ cells/well and incubated overnight in media supplemented with 10% FBS followed by treatment with various concentrations of isopsoralen. After 24h of incubation, cells are washed with phosphate-buffered saline (PBS) and then treated with media containing 100 μg/ml MTT for 2 h at 37℃. The cells are then washed with PBS, and dissolved in 200 μl of DMSO. The resulting solubilized purple formazan is quantified by measuring the absorbance at a wavelength of 540 nm using a spectrophotometer.

Animal Study:^{^[1]}

Animal Models
ICR mice
Dosages
10 mg/kg and 20 mg/kg
Administration
intragastrically

References

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SHIPPING AND STORAGE
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