Iso-Steviol

Catalog No.S3905 Batch:S390501

Technical Data

Formula	C₂₀H₃₀O₃
Molecular Weight	318.45	CAS No.	27975-19-5
Solubility (25°C)*	In vitro	DMSO	63 mg/mL (197.83 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Isosteviol (ISV) is mainly obtained by acid hydrolysis of stevioside, the sweet food additive extracted from the plant Stevia Rebaudiana Bertoni (Bertoni). It possesses various biological activities including anti-hyperglycemic, anti-hypertensive, anti-tumor, anti-inflammatory, and antioxidant effects.
In vivo	Isosteviol (ISV) improves glucose and insulin sensitivity, lowers plasma triglycerides, lowers weight in diabetic KKAy mice, and markedly changes the gene expression profile of key insulin regulatory genes. ISV has a high bioavailability and a potent insulinotropic effect^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines INS-1E Cells Concentrations 0.1 μM Incubation Time 72 h Method NS-1E cells are seeded in 96-well plates at a density of 3 × 10⁴ cells/well in 100 μL medium. The cells are allowed to adhere overnight. Thereafter, they are treated and cultured with different concentrations of glucose, palmitic acid, and leucine with/without 10⁻⁷ M ISV or 10⁻⁷ M Glucagon-like peptide-1 (GLP-1). After 72 h, the number of dead cells in each well is calculated using a fluorometric assasy kit based on the cell lysis and staining method.

Cell Assay:^{^[1]}

Cell lines
INS-1E Cells
Concentrations
0.1 μM
Incubation Time
72 h
Method
NS-1E cells are seeded in 96-well plates at a density of 3 × 10⁴ cells/well in 100 μL medium. The cells are allowed to adhere overnight. Thereafter, they are treated and cultured with different concentrations of glucose, palmitic acid, and leucine with/without 10⁻⁷ M ISV or 10⁻⁷ M Glucagon-like peptide-1 (GLP-1). After 72 h, the number of dead cells in each well is calculated using a fluorometric assasy kit based on the cell lysis and staining method.

References

[1] Wenqian Gu, et al. Nutrients 2018, 10(2), 127.

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.