Imperatorin

Catalog No.S3809 Batch:S380901

Technical Data

Formula	C₁₆H₁₄O₄
Molecular Weight	270.28	CAS No.	482-44-0
Solubility (25°C)*	In vitro	DMSO	54 mg/mL (199.79 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Imperatorin (Ammidin, Marmelosin, Pentosalen, 8-Isoamylenoxypsoralen), a naturally occurring psoralen-type furanocoumarin, is a potent myorelaxant agent and acts as a calcium antagonist on vascular smooth muscle.
In vitro	Imperatorin has estrogen-like activities in MCF-7 cells and stimulatory effects on ALP activity of Saos-2 osteoblast cells^[1].
In vivo	Imperatorin acutely administered at the doses of 10 and 20 mg/kg impairs anxiogenic effect of nicotine in the elevated plus maze (EPM) test. Imperatorin acts as irreversible inactivator of GABA-transaminase and increases the GABA content in the brain. Co-administration of subthreshold doses of imperatorin and nicotine produces an improvement inmemory processes in animals^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines MCF-7 cells Concentrations 0.1 μmol/L Incubation Time 24 h Method The MCF-7 cells are seeded into six-well plates in standard growth medium at a density of 100 000 cells per well and allowed to attach overnight (24 h). The growth medium is replaced with phenol red free DMEM containing 10% charcoal-dextran stripped human serum (hormone-free) for 72 h, after which the medium is refreshed and chemicals are added. After a further 24-h exposure, the cells are harvested and washed with PBS. Cell pellets, which are obtained by centrifugation at 1000 rpm for 15 minutes, are stained with 1 mL of freshly propidium iodide (PI) staining solution (0.1% of Triton X-100, 0.1% of sodium citrate, 50 μg/mL of PI and 10 μg/mL of RNase A in PBS). Flow cytometric analysis of cell cycle distribution is performed.
Animal Study:^{^[2]}	Animal Models male Swiss mice Dosages 1, 10 and 20 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
MCF-7 cells
Concentrations
0.1 μmol/L
Incubation Time
24 h
Method
The MCF-7 cells are seeded into six-well plates in standard growth medium at a density of 100 000 cells per well and allowed to attach overnight (24 h). The growth medium is replaced with phenol red free DMEM containing 10% charcoal-dextran stripped human serum (hormone-free) for 72 h, after which the medium is refreshed and chemicals are added. After a further 24-h exposure, the cells are harvested and washed with PBS. Cell pellets, which are obtained by centrifugation at 1000 rpm for 15 minutes, are stained with 1 mL of freshly propidium iodide (PI) staining solution (0.1% of Triton X-100, 0.1% of sodium citrate, 50 μg/mL of PI and 10 μg/mL of RNase A in PBS). Flow cytometric analysis of cell cycle distribution is performed.

Animal Study:^{^[2]}

Animal Models
male Swiss mice
Dosages
1, 10 and 20 mg/kg
Administration
i.p.

References

Selleck's Imperatorin has been cited by 5 publications

Imperatorin Restores Chemosensitivity of Multidrug-Resistant Cancer Cells by Antagonizing ABCG2-Mediated Drug Transport [ Pharmaceuticals (Basel), 2023, 10.3390/ph16111595]	PubMed: 38004460
Coumarins of Lovage Roots (Levisticum officinale W.D.J.Koch): LC-MS Profile, Quantification, and Stability during Postharvest Storage [ Metabolites, 2022, 13(1)3]	PubMed: 36676932
Direct Targeting of CREB1 with Imperatorin Inhibits TGF β 2-ERK Signaling to Suppress Esophageal Cancer Metastasis [ Adv Sci (Weinh), 2020, 7(16):2000925]	PubMed: 32832354
Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function [ Front Cell Dev Biol, 2020, 8:592433]	PubMed: 33409275
Imperatorin improves in vitro porcine embryo development by reducing oxidative stress and autophagy. [ Theriogenology, 2019, 10.1016/j.theriogenology]	PubMed: 31831188

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.