Idoxuridine

Catalog No.S1883 Batch:S188301

Technical Data

Formula	C₉H₁₁IN₂O₅
Molecular Weight	354.1	CAS No.	54-42-2
Solubility (25°C)*	In vitro	DMSO	35 mg/mL (98.84 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Idoxuridine (NSC 39661, SKF 14287,5-Iodo-2′-deoxyuridine,5-IUdR,IdUrd) is an antiviral agent for feline herpesvirus type-1 with IC50 of 4.3 μM.

Targets

Feline herpesvirus type-1(FHV-1) ^[1]

4.3 μM

In vitro

Idoxuridine is a nucleoside analogue, a modified form of deoxyuridine, similar enough to be incorporated into viral DNA replication, but the iodine atom added to the uracil component blocks base pairing.Idoxuridine is used for topical treatment of herpetic eye infections due to herpes simplex virus (HSV)^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Cultured Crandell-Reese feline kidney (CRFK) cells and FHV-1 strain 727. Concentrations 5, 10, and 50 µM Incubation Time 24, 48, and 72 h Method The CRFK cells are cultured in 25-cm2 flasks that contained DMEM with 10% FBS. The concentration of idoxuridine is 5, 10, and 50µM. Cells from 1 control flask and 1 flask containing each drug concentration are examined by use of an inverted microscope to detect morphologic changes and evaluate confluence, and cells are then harvested at 24, 48, and 72 hours. Culture medium is decanted, and the monolayer is rinsed once with PBS solution. Cells are then enzymatically detached with trypsin-EDTA solution. Trypsinization is stopped by the addition of DMEM with 10% FBS, and cells are forcefully pipetted to ensure complete detachment from the flasks. Each flask is then rinsed once with DMEM with 10% FBS, and the eluent is combined with the first cell suspension to ensure collection of as many cells as possible from each flask. Cell-containing medium is centrifuged (300 X g for 6 minutes), and the supernatant is decanted. Cells are then resuspended in a known volume of DMEM and counted on a hemacytometer. Cells cultured in each drug concentration at each time point are counted twice, and the total number of cells in each flask is calculated.

Cell Assay:^{^[1]}

Cell lines
Cultured Crandell-Reese feline kidney (CRFK) cells and FHV-1 strain 727.
Concentrations
5, 10, and 50 µM
Incubation Time
24, 48, and 72 h
Method
The CRFK cells are cultured in 25-cm2 flasks that contained DMEM with 10% FBS. The concentration of idoxuridine is 5, 10, and 50µM. Cells from 1 control flask and 1 flask containing each drug concentration are examined by use of an inverted microscope to detect morphologic changes and evaluate confluence, and cells are then harvested at 24, 48, and 72 hours. Culture medium is decanted, and the monolayer is rinsed once with PBS solution. Cells are then enzymatically detached with trypsin-EDTA solution. Trypsinization is stopped by the addition of DMEM with 10% FBS, and cells are forcefully pipetted to ensure complete detachment from the flasks. Each flask is then rinsed once with DMEM with 10% FBS, and the eluent is combined with the first cell suspension to ensure collection of as many cells as possible from each flask. Cell-containing medium is centrifuged (300 X g for 6 minutes), and the supernatant is decanted. Cells are then resuspended in a known volume of DMEM and counted on a hemacytometer. Cells cultured in each drug concentration at each time point are counted twice, and the total number of cells in each flask is calculated.

References

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.