ID-8

Catalog No.S7327 Batch:S732701

Technical Data

Formula	C₁₆H₁₄N₂O₄
Molecular Weight	298.29	CAS No.	147591-46-6
Solubility (25°C)*	In vitro	DMSO	60 mg/mL (201.14 mM)
		Ethanol	2 mg/mL (6.7 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

ID-8 is a DYRK inhibitor, and sustains embryonic stem cell self-renewal in long-term culture.

Targets

DYRK ^[1]

In vitro

ID-8 reversibly maintains the long-term culture of ESCs by sustaining Nanog gene expression through Sox2-Oct3/4 activation. ^[1] ID-8 enhances Wnt-mediated hESC survival and proliferation via inhibition of DYRKs. The mechanism study indicates that ID-8 maintains the undifferentiated state in the presence of Wnt by modulating Wnt/β-catenin signaling and enhancing the CBP/β-catenin association in hESCs. ^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines Human embryonic stem cells Concentrations ~10 μM Incubation Time 7 days Method Briefly, the hESCs in feeder-free culture are dissociated completely with 0.05% trypsin-EDTA and seeded at 104 cells per well in Matrigel-coated 6-well culture plates and cultured in MEF-CM. Various concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are supplemented into the culture media at the onset of seeding and then continuously until the end of culturing. For all assays, the cell and colony morphology are examined under a microscope, and the replating efficiency is examined by counting the number of colonies after 7 days of culture.

Cell Assay:^{^[2]}

Cell lines
Human embryonic stem cells
Concentrations
~10 μM
Incubation Time
7 days
Method
Briefly, the hESCs in feeder-free culture are dissociated completely with 0.05% trypsin-EDTA and seeded at 104 cells per well in Matrigel-coated 6-well culture plates and cultured in MEF-CM. Various concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are supplemented into the culture media at the onset of seeding and then continuously until the end of culturing. For all assays, the cell and colony morphology are examined under a microscope, and the replating efficiency is examined by counting the number of colonies after 7 days of culture.

References

Selleck's ID-8 has been cited by 1 publication

In vivo drug discovery for increasing incretin-expressing cells identifies DYRK inhibitors that reinforce the enteroendocrine system [ Cell Chem Biol, 2022, 29(9):1368-1380.e5]	PubMed: 35998625

In vivo drug discovery for increasing incretin-expressing cells identifies DYRK inhibitors that reinforce the enteroendocrine system [ Cell Chem Biol, 2022, 29(9):1368-1380.e5]

PubMed: 35998625

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SHIPPING AND STORAGE
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