ICCB-19 hydrochloride

Catalog No.S6078 Batch:S607801

Technical Data

Formula	C₁₂H₂₂ClN₃OS
Molecular Weight	291.84	CAS No.	1803605-68-6
Solubility (25°C)*	In vitro	DMSO	58 mg/mL (198.73 mM)
		Water	58 mg/mL (198.73 mM)
		Ethanol	58 mg/mL (198.73 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

ICCB-19 hydrochloride is an inhibitor of TNFRSF1A Associated Via Death Domain (TRADD) with IC50 of 1.12 μM and 2.01 μM for protecting Velcade-induced apoptosis in Jurkat cells and protecting RDA in MEFs, respectively. ICCB-19 indirectly inhibits Receptor-interacting serine/threonine-protein kinase 1 (RIPK1). ICCB-19 effectively induces autophagy.

Targets

TRADD ^[1]	RIPK1 ^[1]

In vitro

ICCB-19 binds to a pocket on the N-terminal TRAF2-binding domain of TRADD (TRADD-N), which interacts with the C-terminal domain (TRADD-C) and TRAF2 to modulate the ubiquitination of RIPK1 and beclin 1.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines HEK293T cells, MEFs, Jurkat cells, SH-SY5Y cells, HT-29 cells, U-937 cells, H4 cells and HeLa cells Concentrations 10 μM Incubation Time -- Method The rates of cell death were measured in triplicate or quadruplicate in a 96-well or 384-well plate using SYTOX Green Nucleic Acid Stain or ToxiLight Non-destructive Cytotoxicity BioAssay Kit. The intensity of luminescence was determined in an EnSpire Multimode Plate Reader. Cytotoxicity was expressed as percentages of cell death per well after deducting the background signal in non-induced cells and compared to that of the maximal cell death with 100% Lysis Reagent.

Cell Assay:^{^[2]}

Cell lines
HEK293T cells, MEFs, Jurkat cells, SH-SY5Y cells, HT-29 cells, U-937 cells, H4 cells and HeLa cells
Concentrations
10 μM
Incubation Time
--
Method
The rates of cell death were measured in triplicate or quadruplicate in a 96-well or 384-well plate using SYTOX Green Nucleic Acid Stain or ToxiLight Non-destructive Cytotoxicity BioAssay Kit. The intensity of luminescence was determined in an EnSpire Multimode Plate Reader. Cytotoxicity was expressed as percentages of cell death per well after deducting the background signal in non-induced cells and compared to that of the maximal cell death with 100% Lysis Reagent.

References

Selleck's ICCB-19 hydrochloride has been cited by 1 publication

Inhibition of TRADD ameliorates chondrocyte necroptosis and osteoarthritis by blocking RIPK1-TAK1 pathway and restoring autophagy [ Cell Death Discov, 2023, 9(1):109]	PubMed: 37002200

Inhibition of TRADD ameliorates chondrocyte necroptosis and osteoarthritis by blocking RIPK1-TAK1 pathway and restoring autophagy [ Cell Death Discov, 2023, 9(1):109]

PubMed: 37002200

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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