4'-Hydroxychalcone

Catalog No.S4937 Batch:S493702

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Technical Data

Formula

C15H12O2
Molecular Weight 224.25 CAS No. 2657-25-2
Solubility (25°C)* In vitro DMSO 44 mg/mL (196.2 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description 4'-Hydroxychalcone (P-Cinnamoylphenol), found in herbs and spices and tea, is a member of the class of compounds known as retrochalcones. It has diverse biological activities, inhibiting TNFα-induced NF-κB pathway activation in a dose-dependent manner and activating BMP signaling.
Targets
NF-κB [2]
In vitro The oxidation to 4′-hydroxychalcone was catalyzed by CYP 1A1 and 2C6. It shows estrogenic activity[1]. 4'-hydroxychalcone inhibits TNFα-induced NF-kB pathway activation in a dose-dependent manner. It inhibits TNFα-dependent degradation of IkBα and subsequently prevents p50/p65 nuclear translocation leading to 40-hydroxychalcone-inhibited expression of NF-kB target genes. Inhibition of NF-kB activation by 4'-hydroxychalcone is not leukemia cell-type specific and has no significant effect on non-transformed cell viability. 26S proteasome is a major molecular target of 4'-hydroxychalcone[2].

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    K562 cells

  • Concentrations

    20, 24, 28, 32, 40 μM

  • Incubation Time

    2 h

  • Method

    K562 cells are treated with 4'-hydroxychalcone at the different concentrations for 2 h followed by a TNFα-treatment (20 ng/mL) during 6 h.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.