Hydroxy Camptothecine

Catalog No.S3898 Batch:S389801

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Technical Data

Formula

C20H16N2O5
Molecular Weight 364.35 CAS No. 64439-81-2
Solubility (25°C)* In vitro DMSO 29 mg/mL (79.59 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Hydroxy Camptothecine (10-Hydroxycamptothecin, Hydroxycamptothecine) is a cell-permeable powerful DNA topoisomerase I inhibitor. It has selective inhibitory effect on the phosphorylation of histone H1 and H3, but less effect on other histones.
Targets
DNA topoisomerase I [1]
In vitro 10-HCPT has an inhibitory effect on phosphorylation of histone H1 and H3 in murine hepatoma cells and also exhibits a differentiation inducing effect in human HepG2 cells. 10-HCPT inhibits the cell growth, reduces the cell viability and disturbs the cell cycle distribution of human colon cancer cell line Colo 205. It induces cell cycle arrest in the G2/M phase[1].
In vivo 10-HCPT can be safely orally administered and, alternatively, a low-dose long-term treatment[1]. Prolonged elimination of HCPT in vivo may have a significant impact on its therapeutic effects. HCPT is metabolized to its carboxylate form and glucuronides. Dose-dependent toxicity is observed with i.v. administration of HCPT[2].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    Colo 205 cells

  • Concentrations

    5, 10, 15 or 20 nM

  • Incubation Time

    24-120 h

  • Method

    Colo 205 cells (5×105) (ATCC:CCL-222) are seeded in 25T flasks overnight and then treated without (control) and with 5, 10, 15 or 20 nM of HCPT, respectively. After treatment for 24-120 h, cells are harvested by trypsin-EDTA and then centrifuged at 1,500 rpm for 5 min at 4˚C. The cell pellet is resuspended in culture medium containing 0.04% trypan blue and the viable cells are enumerated by a hemocytometer.
Animal Study:[1]
  • Animal Models

    BALB/c-nude mice

  • Dosages

    1, 2.5, 5, 7.5 mg/kg

  • Administration

    oral administration

Selleck's Hydroxy Camptothecine has been cited by 1 publication

Decreasing Arl4c expression by inhibition of AKT signal in human lung adenocarcinoma cells. [ Life Sci, 2020, 246:117428] PubMed: 32057901

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.