HSF1A

Catalog No.S0294 Batch:S029402

Technical Data

Formula	C₂₁H₁₉N₃O₂S₂
Molecular Weight	409.52	CAS No.	1196723-93-9
Solubility (25°C)*	In vitro	DMSO	82 mg/mL (200.23 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	HSF1A is a cell-permeable heat shock transcription factor 1 (HSF1) activator. HSF1A protects cells from stress-induced apoptosis, binds TRiC subunits and inhibits TRiC activity without perturbation of ATP hydrolysis.
In vitro	HSF1A, an HSF1 activator, alleviates DOX-induced cardiomyocyte apoptosis via suppression of the IGF-IIR apoptotic signaling pathway.^[1]
In vivo	HSF1A enhances HSF1 activity, stabilizes HSF1 expression and minimizes Doxorubicin (DOX)-induced cardiac damage. WKY rats are challenged with DOX (accumulated dose: 30 mg/kgw), and DOX combined with HSF1A. Supplementation with HSF1A significantly elevates cardiac functions back to the levels of the control group. HSF1A has been shown to stimulate human HSF1 nuclear translocation, elevate protein chaperone expression and ameliorate protein misfolding and cell death in a neurodegenerative disease model. The echocardiographic results show that HSF1A also alleviates DOX-induced failures in cardiac function.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines H9c2 cells, Neonatal rat ventricular myocytes (NRVMs) Concentrations 10 μM Incubation Time 24 h Method After treatments of HSF1A for 24 h, H9c2 cells and NRVMs are fixed with a 4% paraformaldehyde solution for 30 min. Following a rinse with PBS, the samples are incubated first with phalloidin–rhodamine for 1 h and subsequently with the TUNEL reaction mixture, containing terminal deoxynucleotidyl transferase and Resorcinolphthalein isothiocyanate-dUTP.

Cell Assay:^{^[1]}

Cell lines
H9c2 cells, Neonatal rat ventricular myocytes (NRVMs)
Concentrations
10 μM
Incubation Time
24 h
Method
After treatments of HSF1A for 24 h, H9c2 cells and NRVMs are fixed with a 4% paraformaldehyde solution for 30 min. Following a rinse with PBS, the samples are incubated first with phalloidin–rhodamine for 1 h and subsequently with the TUNEL reaction mixture, containing terminal deoxynucleotidyl transferase and Resorcinolphthalein isothiocyanate-dUTP.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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