Homosalate

Catalog No.S4572 Batch:S457201

Technical Data

Formula	C₁₆H₂₂O₃
Molecular Weight	262.34	CAS No.	118-56-9
Solubility (25°C)*	In vitro


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description	Homosalate (HMS, Homomenthyl salicylate) is an organic ultraviolet filter used in most sunscreens but has been reported to be toxic to marine organisms. Homosalate aggravates the invasion of human trophoblast cells as well as regulates intracellular signaling pathways including PI3K/AKT and MAPK pathways.
In vitro	Extracellular vesicles (EVs) released by Homosalate‐treated cells enhance resistance to anchorage loss in another recipient epithelial tumour cell line.^[2]
Density	1.045 g/mL

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines MDA‐MB‐231 parental cells Concentrations 10 μM Incubation Time 24 h Method For EV isolation, 6 × 10⁶ of MDA‐MB‐231 parental cells were seeded per 15 cm culture dish in an optimized number of dishes to obtain 70 × 10⁶, 27 × 10⁶ or 50 × 10⁶ secreting cells. After 24 h, cells were washed 1 X with PBS and 15 ml of serum free, phenol red free Leibovitz's L‐15 were added. DMSO (0.1%), Homosalate (10 μM) or Bafilomycin A1 (100 nM) were added to phenol‐red free, serum‐free Leibovitz's L‐15 medium incubated on cells during 24 h (DMSO and Homosalate) or 16 h (Bafilomycin A1). The day after, cells were counted and the percentage of cell viability was determined using Trypan Blue.

Cell Assay:^{^[2]}

Cell lines
MDA‐MB‐231 parental cells
Concentrations
10 μM
Incubation Time
24 h
Method
For EV isolation, 6 × 10⁶ of MDA‐MB‐231 parental cells were seeded per 15 cm culture dish in an optimized number of dishes to obtain 70 × 10⁶, 27 × 10⁶ or 50 × 10⁶ secreting cells. After 24 h, cells were washed 1 X with PBS and 15 ml of serum free, phenol red free Leibovitz's L‐15 were added. DMSO (0.1%), Homosalate (10 μM) or Bafilomycin A1 (100 nM) were added to phenol‐red free, serum‐free Leibovitz's L‐15 medium incubated on cells during 24 h (DMSO and Homosalate) or 16 h (Bafilomycin A1). The day after, cells were counted and the percentage of cell viability was determined using Trypan Blue.

References

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.