Homoharringtonine (HHT)

Catalog No.S9015 Batch:S901502

Technical Data

Formula	C₂₉H₃₉NO₉
Molecular Weight	545.62	CAS No.	26833-87-4
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (183.27 mM)
		Ethanol	100 mg/mL (183.27 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Homoharringtonine (HHT), a plant alkaloid with antitumor properties, inhibits protein translation by preventing the initial elongation step of protein synthesis via an interaction with the ribosomal A-site. Homoharringtonine reversiblely inhibits IL-6-induced STAT3 Tyrosine 705 phosphorylation and reduced anti-apoptotic proteins expression.
In vitro	Homoharringtonine (HHT), a plant alkaloid with antitumor properties, induces p62-mediated autophagy in resistant CML K562G cells, thus promoting autophagic degradation of the BCR-ABL protein.^[1]
In vivo	Homoharringtonine (HHT) in combination with ABT-199, significantly prolongs overall survival of primary diffuse large B-cell lymphoma (DLBCL) xenograft-bearing mice compared with single-agent approaches.^[2]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines K562 cells, K562G cells Concentrations 100, 50, 25, 12.5, 6.2, 3.1, 1.56, 0.78 and 0.39 ng/ml Incubation Time 48 h Method K562, K562G, and siRNA-K562G cells are seeded in 384-well plates at a density of 8,000 cells/well, and then treated with 0.1% DMSO (vehicle control) or HHT at concentrations of 100, 50, 25, 12.5, 6.2, 3.1, 1.56, 0.78 and 0.39 ng/ml for 48 h. According to the manufacturer's instructions, cell proliferation is assayed using an MTS assay, and the absorbance is measured.
Animal Study:^{^[2]}	Animal Models Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ mice of xenograft models of DLBCL Dosages 50 µg/dose Administration i.p.

Cell Assay:^{^[1]}

Cell lines
K562 cells, K562G cells
Concentrations
100, 50, 25, 12.5, 6.2, 3.1, 1.56, 0.78 and 0.39 ng/ml
Incubation Time
48 h
Method
K562, K562G, and siRNA-K562G cells are seeded in 384-well plates at a density of 8,000 cells/well, and then treated with 0.1% DMSO (vehicle control) or HHT at concentrations of 100, 50, 25, 12.5, 6.2, 3.1, 1.56, 0.78 and 0.39 ng/ml for 48 h. According to the manufacturer's instructions, cell proliferation is assayed using an MTS assay, and the absorbance is measured.

Animal Study:^{^[2]}

Animal Models
Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ mice of xenograft models of DLBCL
Dosages
50 µg/dose
Administration
i.p.

References

Selleck's Homoharringtonine (HHT) has been cited by 19 publications

Comprehensive Analysis of the Tumour Immune Microenvironment in Canine Urothelial Carcinoma Reveals Immunosuppressive Mechanisms Induced by the COX-Prostanoid Cascade [ Vet Comp Oncol, 2024, 10.1111/vco.12999]	PubMed: 39179510
USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473]	PubMed: 37833415
USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473]	PubMed: 37833415
TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment [ iScience, 2023, 26(4):106543]	PubMed: 37123244
Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency [ Autophagy, 2022, 1-19]	PubMed: 35287545
The therapeutic effect of KSP inhibitors in preclinical models of cholangiocarcinoma [ Cell Death Dis, 2022, 13-9:799]	PubMed: 36123339
A survey of optimal strategy for signature-based drug repositioning and an application to liver cancer [ Elife, 2022, 11e71880]	PubMed: 35191375
SiCoDEA: A Simple, Fast and Complete App for Analyzing the Effect of Individual Drugs and Their Combinations [ Biomolecules, 2022, 12(7)904]	PubMed: 35883460
Establishment and Characterization of NCC-PMP1-C1: A Novel Patient-Derived Cell Line of Metastatic Pseudomyxoma Peritonei [ J Pers Med, 2022, 12(2)258]	PubMed: 35207746
Homoharringtonine Attenuates Dextran Sulfate Sodium-Induced Colitis by Inhibiting NF-κB Signaling [ Mediators Inflamm, 2022, 2022:3441357]	PubMed: 36211988

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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