1,2,3,4,5,6-Hexabromocyclohexane

Catalog No.S5902 Batch:S590201

Technical Data

Formula	C₆H₆Br₆
Molecular Weight	557.54	CAS No.	1837-91-8
Solubility (25°C)*	In vitro	DMSO	5 mg/mL (8.96 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

1,2,3,4,5,6-Hexabromocyclohexane (NSC7908) is a potent inhibitor of JAK2 tyrosine kinase autophosphorylation with IC50 value to be estimated in low micromolar range.

Targets

JAK2 ^[1]

In vitro

1,2,3,4,5,6-Hexabromocyclohexane inhibits Jak2 autophosphorylation in a time- and dose-dependent manner. 1,2,3,4,5,6-Hexabromocyclohexane is noncytotoxic to cells at concentrations that maximally inhibit Jak2 tyrosine autophosphorylation^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines BSC-40 cells Concentrations 50 μM Incubation Time 0, 1, 4, or 16 h Method BSC-40 cells are transfected/infected. Prior to cell lysis, 50 µM 1,2,3,4,5,6-hexabromocyclohexane is applied to the cells for 0, 1, 4, or 16 h. Cellular lysates are then prepared, immunoprecipitated with anti-Jak2 antibody, and immunoblotted with anti-phosphotyrosine antibody to measure tyrosine phosphorylated Jak2 levels.

Cell Assay:^{^[1]}

Cell lines
BSC-40 cells
Concentrations
50 μM
Incubation Time
0, 1, 4, or 16 h
Method
BSC-40 cells are transfected/infected. Prior to cell lysis, 50 µM 1,2,3,4,5,6-hexabromocyclohexane is applied to the cells for 0, 1, 4, or 16 h. Cellular lysates are then prepared, immunoprecipitated with anti-Jak2 antibody, and immunoblotted with anti-phosphotyrosine antibody to measure tyrosine phosphorylated Jak2 levels.

References

[1] Sandberg EM, et al. J Med Chem. 2005, 48(7):2526-33.

Selleck's 1,2,3,4,5,6-Hexabromocyclohexane has been cited by 1 publication

CXCL10/CXCR3 signaling mediates inhibitory action by interferon-gamma on CRF-stimulated adrenocorticotropic hormone (ACTH) release. [ Cell Tissue Res, 2016, 364(2):395-404]	PubMed: 26572542

CXCL10/CXCR3 signaling mediates inhibitory action by interferon-gamma on CRF-stimulated adrenocorticotropic hormone (ACTH) release. [ Cell Tissue Res, 2016, 364(2):395-404]

PubMed: 26572542

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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