HA15

Catalog No.S8299 Batch:S829901

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Technical Data

Formula

C23H22N4O3S2
Molecular Weight 466.58 CAS No. 1609402-14-3
Solubility (25°C)* In vitro DMSO 93 mg/mL (199.32 mM)
Ethanol 45 mg/mL (96.44 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
0.25mg/ml Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
0.25mg/ml Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description HA15 is a molecule that targets specifically HSPA5 (Heat shock 70kDa protein 5) also known as BiP (Immunoglobulin heavy-chain-binding protein) or Grp78 (glucose-regulated protein 78). HA15 displays anti-cancerous activity on all melanoma cells tested, including cells isolated from patients and cells that developed resistance to BRAF inhibitors.
Targets
BiP/GRP78/HSPA5 [1]
In vitro HA15 induces ER stress leading to cancer cell death in vitro and overcomes BRAF inhibitor resistance in melanoma cells[1].
In vivo HA15 induces ER stress leading to cancer cell death and exhibits strong efficacy in xenograft mouse models with melanoma cells either sensitive or resistant to BRAF inhibitors[1].

Protocol (from reference)

Customer Product Validation

Data from [Data independently produced by , , Cancer Science, 2018, 109:373-383]

Selleck's HA15 has been cited by 14 publications

Dormant cancer cells and polyploid giant cancer cells: The roots of cancer recurrence and metastasis [ Clin Transl Med, 2024, 14(2):e1567] PubMed: 38362620
Temozolomide Sensitizes ARID1A-Mutated Cancers to PARP Inhibitors [ Cancer Res, 2023, 83(16):2750-2762] PubMed: 37306706
Depletion of HIV reservoir by activation of ISR signaling in resting CD4+T cells [ iScience, 2023, 26(1):105743] PubMed: 36590168
Ibrutinib in the Treatment of Solid Tumors: Current State of Knowledge and Future Directions [ Front Oncol, 2022, 12:842418] PubMed: 35299733
Suppressing phosphoinositide-specific phospholipases Cγ1 promotes mineralization of osteoarthritic subchondral bone osteoblasts via increasing autophagy, thereby ameliorating articular cartilage degeneration [ Bone, 2022, 154:116262] PubMed: 34813965
Linking unfolded protein response to ovarian cancer cell fusion [ BMC Cancer, 2022, 22(1):622] PubMed: 35672715
Castration-Induced Downregulation of SPARC in Stromal Cells Drives Neuroendocrine Differentiation of Prostate Cancer [ Cancer Res, 2021, 81(16):4257-4274] PubMed: 34185677
Cell Proliferation Is Strongly Associated with the Treatment Conditions of an ER Stress Inducer New Anti-Melanoma Drug in Melanoma Cell Lines [ Biomedicines, 2021, 9(2)E96] PubMed: 33498201
Multifunctional Graphene-Oxide-Reinforced Dissolvable Polymeric Microneedles for Transdermal Drug Delivery [ ACS Appl Mater Interfaces, 2020, 12(1):352-360] PubMed: 31825580
The stabilization of PD-L1 by the endoplasmic reticulum stress protein GRP78 in triple-negative breast cancer [ Am J Cancer Res, 2020, 10(8):2621-2634] PubMed: 32905506

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.