GsMTx4

Catalog No.P1205 Batch:P120501

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Technical Data

Formula

C185H273N49O45S6
Molecular Weight 4095.86 CAS No. 1209500-46-8
Solubility (25°C)* In vitro Water 100 mg/mL (24.41 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description GsMTx4 (Grammostola spatulata mechanotoxin 4, M-theraphotoxin-Gr1a, M-TRTX-Gr1a) is a spider venom peptide that inhibits cationic mechanosensitive channels (MSCs).
Targets
MSCs [1]
In vitro

Pretreatment of GsMTx4 and BAPTA-AM suppress the Yoda1-induced accumulation of HIF-1alpha, NFATC1, and NFATC3 proteins and also suppress Yoda1-induced VEGF-A expression at the mRNA and protein levels.[2]
Density 95.0~105.0%

Protocol (from reference)

Cell Assay:

[3]
  • Cell lines

    BMDMs, RAW264.7 cells

  • Concentrations

    5, 10 µM

  • Incubation Time

    30 min

  • Method

    To study the effect of mechanical stretching, BMDMs or RAW264.7 cells were treated with Yoda1, with or without pretreatment with inhibitors for 30 min. The inhibitors included cyclosporin A, FK506, echinomycin, GsMTx4, BAPTA-AM and RN-1734. In some experiments, BMDMs were treated with GSK1016790A.

    (Data sourced from selleck products)
Animal Study:

[3]
  • Animal Models

    Male C57BL/6 mice

  • Dosages

    0.8 mg/kg

  • Administration

    i.p.

Selleck's GsMTx4 has been cited by 7 publications

Low-intensity pulsed ultrasound promotes the osteogenesis of mechanical force-treated periodontal ligament cells via Piezo1 [ Front Bioeng Biotechnol, 2024, 12:1347406] PubMed: 38694622
Low-intensity pulsed ultrasound promotes the osteogenesis of mechanical force-treated periodontal ligament cells via Piezo1 [ Front Bioeng Biotechnol, 2024, 12:1347406] PubMed: 38694622
Piezo1 promotes peripheral nerve fibrotic scar formation through Schwann cell senescence [ Neurosci Lett, 2024, 837:137916] PubMed: 39059459
Enriched environment ameliorates fear memory impairments induced by sleep deprivation via inhibiting PIEZO1/calpain/autophagy signaling pathway in the basal forebrain [ CNS Neurosci Ther, 2023, 10.1111/cns.14365] PubMed: 37485782
An EPHB4-RASA1 signaling complex inhibits shear stress-induced Ras-MAPK activation in lymphatic endothelial cells to promote the development of lymphatic vessel valves [ bioRxiv, 2023, 2023.11.22.568378] PubMed: 38045382
Piezo1-mediated mechanosensation in bone marrow macrophages promotes vascular niche regeneration after irradiation injury [ Theranostics, 2022, 12(4): 1621-1638] PubMed: N/A
Piezo1-mediated mechanosensation in bone marrow macrophages promotes vascular niche regeneration after irradiation injury [ Theranostics, 2022, 12(4):1621-1638] PubMed: 35198061

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.