GSK2200150A

Catalog No.S0423 Batch:S042301

Technical Data

Formula	C₂₀H₂₃NO₃S
Molecular Weight	357.47	CAS No.	1443138-53-1
Solubility (25°C)*	In vitro	DMSO	71 mg/mL (198.61 mM)
		Ethanol	18 mg/mL (50.35 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

GSK2200150A is identified by high-throughput screening (HTS) campaign that can be used as an anti-tuberculosis (TB) agent (MIC = 0.38 µM).

Targets

tuberculosis ^[1]

In vitro

GSK2200150A is a novel antimycobacterial agent against Mycobacterium tuberculosis, by inhibiting the growth of virulent M.tuberculosis strain (H37Rv) with MIC of 0.38 μM.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines human monocytic cell line (THP1 cells) Concentrations 1.56–200 µM Incubation Time 4 days Method THP1 cells (1×10⁵) in media containing 50 ng/mL phorbol 12-myristate 13-acetate (PMA) are added to 96-well plates and are then incubated for 48 h at 37°C to allow adherence and differentiation. GSK2200150A (1.56–200 µM) is added to the wells and incubated for 4 days at 37°C. Then 0.05%(w/v) resazurin (4 h) is added and the fluorescence measured. Cell viability is calculated as percentage fluorescence in comparison to untreated cells.

Cell Assay:^{^[1]}

Cell lines
human monocytic cell line (THP1 cells)
Concentrations
1.56–200 µM
Incubation Time
4 days
Method
THP1 cells (1×10⁵) in media containing 50 ng/mL phorbol 12-myristate 13-acetate (PMA) are added to 96-well plates and are then incubated for 48 h at 37°C to allow adherence and differentiation. GSK2200150A (1.56–200 µM) is added to the wells and incubated for 4 days at 37°C. Then 0.05%(w/v) resazurin (4 h) is added and the fluorescence measured. Cell viability is calculated as percentage fluorescence in comparison to untreated cells.

References

[1] Badiola KA, et al. PLoS One. 2014 Dec 10;9(12):e111782.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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