Glycitin

Catalog No.S3825 Batch:S382501

Technical Data

Formula	C₂₂H₂₂O₁₀
Molecular Weight	446.40	CAS No.	40246-10-4
Solubility (25°C)*	In vitro	DMSO	89 mg/mL (199.37 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Glycitin (Glycitein-7-β-O-glucoside), a natural isoflavone isolated from legumes, has antibacterial, antiviral and estrogenic activities and may exerts preventative effects on alcoholism, cardiovascular and cerebrovascular diseases and some types of cancer.
In vitro	Glycitin promotes cell proliferation, osteoblast induction, and activates Col I mRNA expression and ALP activity of BMSCs. Glycitin promotes the proliferation and migration of human dermal fibroblast cells through TGF-β and p-AKT signaling^[1].
In vivo	Glycitin significantly prevents bone loss in variectomized (ovx) rats at a dose of 50 mg/kg/d. At this dose glycitin also prevents ovx-induced uterine atrophy and increases in body weight gain, abdominal fat, serum total cholesterol and triglyceride, and urinary excretion of pyridinoline and deoxypyridinoline with statistical significance^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Bone marrow stem cells (BMSCs) Concentrations 0.01, 0.5, 1, 5 and 10 µM Incubation Time 7 days Method BMSCs (1-2×10⁶ cells or 1-2×10⁴ per well) are cultured in 6- or 96-well culture plates overnight at 37°C in an atmosphere containing 5% CO₂. Glycitin is added to the wells at final concentrations of 0.01, 0.5, 1, 5 and 10 µM and cultured for 7 days. In cells cultured in 6-well culture plates, BMSCs are determined using Oil Red O staining and observed via light microscopy at 510 nm. BMSCs are fixed using 5% precooled paraformaldehyde for 30 min at 4°C and stained with 0.6% (w/v) Oil Red O solution for 15 min at room temperature. Cells stained with Oil Red O are washed with water (3×5 min) to remove unbound dye, and culture dishes are stained with 1 ml isopropyl alcohol for 10 min. In cells cultured in 96-well culture plates, BMSCs are determined via MTT assay. A total of 20 µl MTT (5 g/l) are added to each well and cultured for 4 h. The supernatant is removed and 200 µl dimethylsulfoxide are added to each well for 15 min. Optical density (OD) is measured using a microplate spectrophotometer at 570 nm. Proliferation rate is calculated using: OD treated / OD control × 100%.
Animal Study:^{^[2]}	Animal Models Female Sprague-Dawley rats Dosages 25, 50 or 100 mg/kg/d Administration oral

Cell Assay:^{^[1]}

Cell lines
Bone marrow stem cells (BMSCs)
Concentrations
0.01, 0.5, 1, 5 and 10 µM
Incubation Time
7 days
Method
BMSCs (1-2×10⁶ cells or 1-2×10⁴ per well) are cultured in 6- or 96-well culture plates overnight at 37°C in an atmosphere containing 5% CO₂. Glycitin is added to the wells at final concentrations of 0.01, 0.5, 1, 5 and 10 µM and cultured for 7 days. In cells cultured in 6-well culture plates, BMSCs are determined using Oil Red O staining and observed via light microscopy at 510 nm. BMSCs are fixed using 5% precooled paraformaldehyde for 30 min at 4°C and stained with 0.6% (w/v) Oil Red O solution for 15 min at room temperature. Cells stained with Oil Red O are washed with water (3×5 min) to remove unbound dye, and culture dishes are stained with 1 ml isopropyl alcohol for 10 min. In cells cultured in 96-well culture plates, BMSCs are determined via MTT assay. A total of 20 µl MTT (5 g/l) are added to each well and cultured for 4 h. The supernatant is removed and 200 µl dimethylsulfoxide are added to each well for 15 min. Optical density (OD) is measured using a microplate spectrophotometer at 570 nm. Proliferation rate is calculated using: OD treated / OD control × 100%.

Animal Study:^{^[2]}

Animal Models
Female Sprague-Dawley rats
Dosages
25, 50 or 100 mg/kg/d
Administration
oral

References

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.