Glucosamine sulfate

Catalog No.S3850 Batch:S385001

Technical Data

Formula	C₆H₁₃NO₅.H₂SO₄
Molecular Weight	277.25	CAS No.	29031-19-4
Solubility (25°C)*	In vitro	Water	55 mg/mL (198.37 mM)
		DMSO	4 mg/mL (14.42 mM)

* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Glucosamine sulfate (D-Glucosaminesulfate, D-Glucosamine sulphate) is a dietary supplement commonly used in the treatment of osteoarthritis. It is a type of glycosaminoglycan, which is a naturally occurring major component of joint cartilage and joint fluid.
In vitro	Glucosamine sulfate exerts a pronounced suppressive effect on MMPs in osteosarcoma cell lines in vitro^[1]. Glucosamine suppresses the activation of T-lymphoblasts and dendritic cells in vitro as well as allogeneic mixed leukocyte reactivity in a dose-dependent manner. Glucosamine has been shown to inhibit nucleic acid and protein biosynthesis and irreversible damage to organelles in tumor cells, but not in normal cells^[3].
In vivo	Glucosamine sulfate attenuates DSS-induced colitis symptoms, possibly by suppressing the NF-κB-mediated TNF-α and IL-1β production and neutrophil activation that contribute to intestinal barrier disruption in colitic mice^[2]. Glucosamine administration prolongs allogeneic cardiac allograft survival in vivo^[3].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines MG-63 and SaOS-2 cells Concentrations 0, 10, 50 and 100 μg/ml Incubation Time 48 h Method Cells are exposed to 0, 10, 50 and 100 μg/ml glucosamine sulfate for 48 h and treatment toxicity is determined through measurement of cell viability and proliferation. Relative gene expression of matrix metalloproteinase (MMP)-2, -3 and -9 is quantified by real-time polymerase chain reaction. Protein levels of MMP-2 and -9 are assessed by ELISA.
Animal Study:^{^[2]}	Animal Models C57BL/6 mice Dosages 0, 0.05, and 0.10% (w/w) Administration oral

Cell Assay:^{^[1]}

Cell lines
MG-63 and SaOS-2 cells
Concentrations
0, 10, 50 and 100 μg/ml
Incubation Time
48 h
Method
Cells are exposed to 0, 10, 50 and 100 μg/ml glucosamine sulfate for 48 h and treatment toxicity is determined through measurement of cell viability and proliferation. Relative gene expression of matrix metalloproteinase (MMP)-2, -3 and -9 is quantified by real-time polymerase chain reaction. Protein levels of MMP-2 and -9 are assessed by ELISA.

Animal Study:^{^[2]}

Animal Models
C57BL/6 mice
Dosages
0, 0.05, and 0.10% (w/w)
Administration
oral

References

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.